Human UCH-L3 Antibody

Catalog #: MAB6008 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6008
MAB6008-SP

Detection of Human UCH‑L3 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human UCH-L3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human UCH-L3 in direct ELISAs. In direct ELISAs and Western blots, no cross-reactivity with recombinant human UCH-L1 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 677710

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human UCH-L3
Glu2-Ala230
Accession # P15374

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

3 µg/mL

See below

Immunoprecipitation

25 µg/mL

Cell lysates spiked with Recombinant Human UCH-L3, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human UCH‑L3 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 3 µg/mL of Mouse Anti-Human UCH-L3 Monoclonal Antibody (Catalog # MAB6008) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for UCH-L3 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: UCH-L3

UCH-L3 (Ubiquitin carboxyterminal hydrolase isozyme 3) is a 26‑28 kDa member of the peptidase C12 family of enzymes. It shows wide expression, being noted in adipocytes, renal duct epithelium, neurons, and striated muscle. UCH‑L3 cleaves both monomeric ubiquitin (Ub) from Ub‑protein conjugates, and a GGLRQ peptide from the C‑terminus of the Ub‑like protein NEDD‑8. Notably, UCH-L3 activity is muted in the presence of Ub interacting dimers. Human UCH-L3 is 230 amino acids (aa) in length. It is phosphorylated on Ser75 and Ser130 and contains two ubiquitin-binding sequences between aa 40‑57 and 178‑186. Four potential splice forms have been reported. Two show a four aa deletion between aa 15‑18, with one also containing a 14 aa substitution for aa 179‑230. Two others contain an alternative start site at Met37, one of which is also accompanied by a seven aa substitution for aa 143‑230. Full length human UCH-L3 shares 98% aa identity with mouse and rat UCH‑L3.

Long Name

Ubiquitin C-terminal Hydrolase L3

Entrez Gene IDs

7347 (Human); 50933 (Mouse); 114094 (Rat); 100350137 (Rabbit)

Alternate Names

EC 3.4.19.12; ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase); ubiquitin carboxyl-terminal hydrolase isozyme L3; Ubiquitin Thioesterase L3; ubiquitin thiolesterase; UCHL3; UCH-L3