Human TXNDC5 Antibody

Catalog #: AF4840 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4840
AF4840-SP

Detection of Human TXNDC5 by Western Blot.

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Human TXNDC5 Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human TXNDC5 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human TXNDC5
Glu110-Leu432
Accession # Q8NBS9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human TXNDC5 by Western Blot. Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, A549 human lung carcinoma cell line, DU145 human prostate carcinoma cell line, and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human TXNDC5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4840) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TXNDC5 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TXNDC5

TXNDC5 (Thioredoxin (TRX) domain-contain protein 5; also ERp46 and EndoPDI) is a 50‑55 kDa member of the disulfide isomerase family of enzymes. It is expressed in hepatocytes, and tumor plus hypoxic endothelium. TXNDC5 demonstrates thioreductase (disulfide bond reduction) activity in the ER. Mature human TXNDC5 is 400 amino acids (aa) in length and migrates anomalously in SDS-PAGE. It contains three TRX domains (aa 36‑428) and a short ER retention motif (aa 429‑432). There are three potential splice variants. One shows an alternate start site at Met109, while two others show a 16 and 45 aa substitution for the N-terminal 88 amino acids. Over aa 110‑432, human TXNDC5 is 88% aa identical to mouse TXNDC5.

Long Name

Thioredoxin Domain Containing 5

Entrez Gene IDs

81567 (Human); 105245 (Mouse)

Alternate Names

EndoPDI; ERp46; FLJ90810; HCC-2; member 15; MGC3178; PDIA15; thioredoxin domain containing 5 (endoplasmic reticulum); thioredoxin domain containing 5; thioredoxin domain-containing protein 5; thioredoxin related protein; TLP46; TXNDC5