Human TSP50 Antibody

Catalog #: AF2430 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2430
AF2430-SP

Detection of Human TSP50 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TSP50 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TSP50 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant mouse TSP50 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TSP50
Ala40-Ala363
Accession # Q9UI38

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human TSP50, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human TSP50 by Western Blot. Western blot shows lysates of human testis tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human TSP50 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2430) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TSP50 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TSP50

TSP50 is a member of the peptidase S1 family of serine proteases. It is a secreted glycoprotein that may play a role in spermatogenesis and act as an oncogene.

Long Name

Testes-specific Protease 50

Entrez Gene IDs

29122 (Human); 235631 (Mouse)

Alternate Names

Cancer/testis antigen 20; CT20probable threonine protease PRSS50; EC 3.4.25.-; protease, serine, 50; Serine protease 50; testes-specific protease 50; Testis-specific protease-like protein 50; TSP50; TSP50testes specific protease 50