Human Tryptase gamma-1/TPSG1 Biotinylated Antibody

Catalog #: BAF1667 Datasheet / COA / SDS

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BAF1667

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Human Tryptase gamma-1/TPSG1 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Tryptase gamma ‑1/TPSG1 in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human (rh) Tryptase alpha -1 and rhTryptase beta -2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Tryptase gamma ‑1/TPSG1
Arg20-Arg281
Accession # Q9NRR2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Tryptase gamma ‑1/TPSG1 (Catalog # 1667-SE)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tryptase gamma-1/TPSG1

As mediators of inflammatory and allergic response, mast cells are found throughout the body concentrated near blood vessels in connective tissue and the mucous membranes of the respiratory and gastrointestinal tract (3). Upon activation, human mast cells release granules that are enriched with neutral serine proteases including Tryptases, chymase and cathepsin G (4). Tryptase gamma -1, also called transmembrane Tryptase, is encoded by TPSG1, one of many serine protease genes clustered in human chromosome 16p13.3 (5). Human Tryptase gamma -1 is synthesized as a 321 amino acid preproenzyme with a C-terminal transmembrane anchor (1, 2). The rhTPSG1 was expressed as a soluble protein terminated at residue 281 and corresponded to the proenzyme. The proenzyme can be cleaved by trypsin to form the active enzyme. The serine protease activity of trypsin-activated rhTPSG1 can be inhibited by ecotin (R&D Systems, Catalog # 1328-PI). Greater than 95% protease activity is inhibited by ecotin at approximately 10:1 molar ratio.

References

Wong, G.W. et al. (1999) J. Biol. Chem. 274:30784.
Caughey, G.H. et al. (2000) J. Immunol. 164:6566.
Harris, J.L. et al. (2001) J. Biol. Chem. 276:34941.
Miller, H.R.P. and A.D. Pemberton (2002) Immunology 105:375.
Wong, G.W. et al. (2002) J. Biol. Chem. 277:41906.

Entrez Gene IDs

25823 (Human)

Alternate Names

EC 3.4.21; EC 3.4.21.-; gamma I; gamma II; lung tryptase; mast cell protease II; mast cell tryptase; PRSS31skin tryptase; Serine protease 31; TMTpituitary tryptase; TPSG1; Transmembrane tryptase; trpA; tryptase gamma 1; tryptase gamma I; tryptase gamma II; tryptase gamma; Tryptase gamma1; Tryptase gamma-1