Human TRPS1 Antibody

Catalog # Availability Size / Price Qty
AF4838
AF4838-SP
Detection of Human TRPS1 by Western Blot.
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Product Details
Citations (5)
FAQs
Supplemental Products
Reviews (1)

Human TRPS1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TRPS1 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human TRPS1
Met1-Phe222
Accession # Q9UHF7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human TRPS1 antibody by Western Blot. View Larger

Detection of Human TRPS1 by Western Blot. Western blot shows lysates of MDA-MB-453 human breast cancer cell line and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TRPS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4838) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TRPS1 at approximately 165 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Simple Western Detection of Human TRPS1 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human TRPS1 by Simple WesternTM. Simple Western lane view shows lysates of MCF-7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for TRPS1 at approximately 191 kDa (as indicated) using 10 µg/mL of Goat Anti-Human TRPS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4838) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRPS1

TRPS1 (also GC79) is a 160-175 kDa (predicted 140 kDa) member of the zinc finger family of transcription factors. It is ubiquitously expressed and serves to repress the expression of GATA-regulated genes. Human TRPS1 is 1281 amino acids (aa) in length. It contains nine divergent zinc-finger motifs (aa 222‑1267), the seventh of which is associated with a GATA-binding sequence (aa 896‑920) and is flanked by two NLSs (aa 886‑891 and 946‑952). Two SUMOylation sites exist (Lys1192 and Lys1201) and when utilized, they each add 40 kDa to the native MW. One alternative splice form shows premature truncation after Lys853. A second shows an alternate start site 13 aa upstream of the standard start site. Over aa 1‑222, human TRPS1 shares 95% and 89% aa identity with canine and mouse TRPS1, respectively.

Long Name
Tricho-rhino-phalangeal Syndrome Type I Protein
Entrez Gene IDs
7227 (Human); 83925 (Mouse); 299897 (Rat)
Alternate Names
GC79; trichorhinophalangeal syndrome I; tricho-rhino-phalangeal syndrome type I protein; TRPS1

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Citations for Human TRPS1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. CHD4 mediates SOX2 transcription through TRPS1 in luminal breast cancer
    Authors: J Zhang, X Lv, B Wei, X Gong, L Chen
    Cellular Signalling, 2022-09-06;100(0):110464.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Atypical GATA transcription factor TRPS1 represses gene expression by recruiting CHD4/NuRD(MTA2) and suppresses cell migration and invasion by repressing TP63 expression
    Authors: Y Wang, X Lin, X Gong, L Wu, J Zhang, W Liu, J Li, L Chen
    Oncogenesis, 2018-12-19;7(12):96.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  3. Transcriptional repressor GATA binding 1-mediated repression of SRY-box 2 expression suppresses cancer stem cell functions and tumor initiation
    Authors: X Gong, W Liu, L Wu, Z Ma, Y Wang, S Yu, J Zhang, H Xie, G Wei, F Ma, L Lu, L Chen
    J. Biol. Chem., 2018-10-12;0(0):.
  4. Tricho-rhino-phalangeal syndrome 1 protein functions as a scaffold required for ubiquitin-specific protease 4-directed histone deacetylase 2 de-ubiquitination and tumor growth
    Authors: Y Wang, J Zhang, L Wu, W Liu, G Wei, X Gong, Y Liu, Z Ma, F Ma, JP Thiery, L Chen
    Breast Cancer Res., 2018-08-02;20(1):83.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. TRPS1 regulates oestrogen receptor binding and histone acetylation at enhancers
    Authors: AA Serandour, H Mohammed, A Miremadi, KW Mulder, JS Carroll
    Oncogene, 2018-06-12;0(0):.
    Species: Human
    Sample Types: Cell Lysates, Complex Sample Type
    Applications: ChIP, Western Blot

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Human TRPS1 Antibody
By Anonymous on 06/26/2018
Application: ChIP Sample Tested: MCF-7 human breast cancer cell line,T47D human breast cancer cell line,ZR-75 human breast cancer cell line Species: Human

https://doi.org/10.1038/s41388-018-0312-2