Human TrkC Antibody

Catalog #: AF373
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF373
AF373-SP

Detection of HAF017 TrkC by Western Blot.

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All TrkC Antibodies

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Citations (4)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TrkC Antibody Summary

Specificity

Detects human TrkC in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human TrkB is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TrkC
Cys32-Asp428
Accession # Q96CY4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

10 µg/mL

See below

Flow Cytometry

0.25 µg/10⁶ cells

Human TrkC transfected Baf/3 cells

Immunohistochemistry

5-15 µg/mL

Immersion fixed paraffin-embedded sections of human brain (hippocampus) and perfusion fixed frozen sections of mouse brain (cortex)

Blockade of Receptor-ligand Interaction

In a functional ELISA, 1-3 µg/mL of this antibody will block 50% of the binding of 5 ng/mL of Recombinant Human NT-3 (Catalog # 267-N3) to immobilized Recombinant Human TrkC Fc Chimera (Catalog # 373-TC) coated at 1 µg/mL (100 µL/well). At 30 μg/mL, this antibody will block >90% of the binding.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of HAF017 TrkC by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue, human brain (hippocampus) tissue, human brain (hypothalamus) tissue, and human brain stem tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TrkC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF373) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for TrkC at approximately 140, 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Human TrkC by Simple Western^TM. Simple Western lane view shows lysates of human brain (hypothalamus) tissue, loaded at 0.2 mg/mL. A specific band was detected for TrkC at approximately 164 kDa (as indicated) using 10 µg/mL of Goat Anti-Human TrkC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF373) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TrkC

The neurotrophins, including NGF, BDNF, NT-3 and NT-4/5, constitute a group of structurally related, secreted proteins that play an important role in the development and function of the nervous system. The biological activities of the neurotrophins are mediated by binding to and activating two unrelated receptor types: the p75 neurotrophin receptor (p75NTR) and the Trk family of receptor tyrosine kinases (1, 2). P75NTR is a member of the tumor necrosis factor receptor superfamily (TNFRSF) and has been designated TNFRSF16. It binds all neurotrophins with low-affinity to transduce cellular signaling pathways that synergize or antagonize those activated by the Trk receptors. Three Trk family proteins, TrkA, TrkB and TrkC, exhibiting different ligand specificities, have been identified. TrkA binds NGF and NT‑3, TrkB binds BDNF, NT-3 and NT-4/5, and TrkC only binds NT-3 (1, 2). All Trk family proteins share a conserved, complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain (3). Natural splice variants of the different Trks, lacking the first cysteine-rich domain, the first and second or all three of the leucine-rich motifs, or the tyrosine kinase domain, have been described (4). At the protein sequence level, Trks are highly conserved between species with the extracellular domains of human and mouse TrkC's showing 94% amino acid sequence identity (5). The proteins also exhibit cross-species activity. The primary location of TrkC expression is in the nervous system and, specifically, in regions of the CNS. Low level TrkC expression has also been observed in a wide variety of tissues outside the nervous system (6).

References

Huang, E.J. and L.F. Reichardt. (2003) Annu. Rev. Biochem. 72:(epub ahead of print).
Dechant, G. (2001) Cell Tissue Res. 305:229.
Schneider, R. and M. Schweiger (1991) Oncogene 6:1807.
Ninkina, N. et al. (1997) J. Biol. Chem. 272:13019.
Menn, B. et al. (1998) J. Comp. Neurol. 401:47.
Shelton, D. et al. (1995) J. Neurosci. 15:477.

Long Name

Neurotrophic Tyrosine Kinase Receptor C

Entrez Gene IDs

4916 (Human); 18213 (Mouse); 29613 (Rat)

Alternate Names

EC 2.7.10; EC 2.7.10.1; ETS related protein-neurotrophic receptor tyrosine kinase fusion protein; ETV6-NTRK3 fusion; gp145(trkC); GP145-TrkC; Neurotrophic tyrosine kinase receptor type 3; neurotrophic tyrosine kinase, receptor, type 3; NT 3 receptor; NT-3 growth factor receptor; NTRK3; TrkC tyrosine kinase; TrkC; trk-C; TRKCneurotrophin 3 receptor; tyrosine kinase receptor C

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human TrkC Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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In vitro modelling of human proprioceptive sensory neurons in the neuromuscular system
Authors: M Badiola-Ma, T Osaki, RD Kamm, J Samitier
Scientific Reports, 2022-12-09;12(1):21318.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Combinatorial analysis of developmental cues efficiently converts human pluripotent stem cells into multiple neuronal subtypes.
Authors: Maury Y, Come J, Piskorowski R, Salah-Mohellibi N, Chevaleyre V, Peschanski M, Martinat C, Nedelec S
Nat Biotechnol, 2014-11-10;33(1):89-96.
Species: Human
Sample Types: Whole Cells
Applications: IHC
TrkB antibody elicits cytotoxicity and suppresses migration/invasion of transitional cell carcinoma cells.
Authors: Huang YT, Lai PC, Wu CC
Int. J. Oncol., 2010-10-01;37(4):943-9.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay
BDNF mediated TrkB activation is a survival signal for transitional cell carcinoma cells.
Authors: Huang YT, Lai PC, Wu CC, Hsu SH, Cheng CC, Lan YF, Chiu TH
Int. J. Oncol., 2010-06-01;36(6):1469-76.
Species: Human
Sample Types: Whole Cells
Applications: ICC