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Human TREML2/TLT-2 Antibody

Catalog #: AF3259
2 Citations 4 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3259
AF3259-SP
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (4)
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TREML2/TLT-2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TREML2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant mouse TREML2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TREML2
Gly19-Ser268
Accession # Q5T2D2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human TREML2/TLT‑2 Fc Chimera

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TREML2/TLT-2

Trem-like transcript 2 protein (TLT-2; also TREML2) is a 33 kDa (unglycosylated) type I transmembrane cell surface receptor and member of the Trem family of receptor proteins.  Human TLT-2 is synthesized as a 321 amino acid (aa) precursor that contains an18 aa signal sequence, a 250 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 32 aa cytoplasmic tail (SwissProt #: Q5T2D2). Within the ECD are an Ig-like V-type domain (aa 20-121) and one potential site of N-linked glycosylation. Mature human TLT-2 shares 49% aa sequence identity with mature mouse TLT-2. Cell surface expression of TLT-2 is seen for most of the B and myeloid cell lines (1, 2). TLT-2 is also expressed constitutively on CD8+ T cells and induced on CD4+ T cells after activation (2). TLT-2 plays a role in the innate and adaptive immune responses. In 2008, Hashiguchi et al. discovered that the B7 family member B7-H3, which also plays important roles in immune responses, specifically bound to TLT-2. According to their research, stimulation with B7-H3 transfectants preferentially up-regulated the proliferation and IFN-gamma production on CD8+ T cells (2). Also, transduction of TLT-2 into T cells resulted in enhanced IL-2 and IFN-gamma production via interactions with B7-H3, and blockade of the B7-H3:TLT-2 pathway with mAb against B7-H3 or TLT-2 efficiently inhibited contact hypersensitivity responses (2). In 2009, however, Leitner et al. extensively analyzed interaction of B7-H3 with TLT-2 and found no evidence for such an interaction (3).

References
  1. King, R.G. et al., 2006, J. Immunol. 176:6012.
  2. Hashiguchi, M. et al., 2008, Proc. Natl. Acad. Sci. USA 105:10495.
  3. Leitner, J. et al., 2009, Eur. J. Immunol. 39:1754.
Long Name
Triggering Receptor Expressed on Myeloid Cells-like 2
Entrez Gene IDs
79865 (Human); 328833 (Mouse)
Alternate Names
C6orf76; dJ238O23.1; FLJ13693; MGC149715; MGC149716; TLT2; TLT-2; TREML2; trem-like transcript 2 protein; triggering receptor expressed on myeloid cells-like 2; triggering receptor expressed on myeloid cells-like protein 2; UNQ6268/PRO20473

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Citations for Human TREML2/TLT-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Generation and Characterization of Two Novel Monoclonal Antibodies Produced Against Human TLT-2 Molecule
    Authors: Jun-Chi Xu, Fei Gao, Feng-Qing Fu, Yong-Jin Chen, Ping Xu, Bin Zhou et al.
    Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
  2. B7-H3 is a potent inhibitor of human T-cell activation: No evidence for B7-H3 and TREML2 interaction.
    Authors: Leitner J, Klauser C, Pickl WF, Stockl J, Majdic O, Bardet AF, Kreil DP, Dong C, Yamazaki T, Zlabinger G, Pfistershammer K, Steinberger P
    Eur. J. Immunol., 2009-07-01;39(7):1754-64.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Human TREML2/TLT-2 Antibody

Average Rating: 4.8 (Based on 4 Reviews)

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Human TREML2/TLT-2 Antibody
By Anonymous on 03/04/2022
Application: WB Sample Tested: 293T human embryonic kidney cell line Species: Human

1st lane: 293T cells + vector,
2nd lane: 293T cells + TLT2 expression vector
exposure time: very short

Western Blot Human TREML2/TLT-2 Antibody AF3259
Human TREML2/TLT-2 Antibody
By Anonymous on 03/01/2022
Application: Flow Sample Tested: 293T human embryonic kidney cell line Species: Human

For the data, it's 293T transfected with hTREML2 plasmid, then stained with this antibody, blue: IgG isotype control, red: antibody AF3259.

Flow Cytometry Human TREML2/TLT-2 Antibody AF3259
Human TREML2/TLT‑2 Antibody
By Anonymous on 10/26/2015
Application: Flow Sample Tested: human peripheral CD11c+ dendritic cells Species: Human

Gated on lymphocyte FSC-A and SSC-A populations. Singlets were further gated on by FSC-A/FSC-H doublet exclusion. After primary antibody staining, cells were wash 3x and Alexa 488 labeled rabbit anti-goat IgG was added for 20 min. Cells were washed 2x and fixed in orthofix. Isotype control shaded grey, TREML2 in open histogram.
Specificity: Specific
Sensitivity: Sensitive
Buffer: 2% human serum, 0.5 mM EDTA in PBS
Dilution: 1/100

Flow Cytometry Human TREML2/TLT‑2 Antibody AF3259
Human TREML2/TLT‑2 Antibody
By Anonymous on 10/26/2015
Application: Flow Sample Tested: human peripheral CD11c+ dendritic cells Species: Human

Gated on lymphocyte FSC-A and SSC-A populations. Singlets were further gated on by FSC-A/FSC-H doublet exclusion. After primary antibody staining, cells were wash 3x and Alexa 488 labeled rabbit anti-goat IgG was added for 20 min. Cells were washed 2x and fixed in orthofix. Isotype control shaded grey, TREML1 in open histogram.
Specificity: Specific
Sensitivity: Sensitive
Buffer: 2% human serum, 0.5 mM EDTA in PBS
Dilution: 1/100

Flow Cytometry Human TREML2/TLT‑2 Antibody AF3259