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Human TRA-1-85/CD147 APC-conjugated Antibody

Catalog #: FAB3195A
17 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB3195A
Detection of TRA‑1‑85/CD147 in Human PBMCs by Flow Cytometry.
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Citations (17)
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Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TRA-1-85/CD147 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human TRA‑1‑85 antigen in flow cytometry.
Source
Monoclonal Mouse IgG1 Clone # TRA-1-85
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
2120Ep human embryonal carcinoma cell line
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of TRA-1-85/CD147 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of TRA‑1‑85/CD147 in Human PBMCs by Flow Cytometry. Human peripheral blood lymphocytes and monocytes were stained with either (A) Mouse Anti-Human TRA-1-85/CD147 APC-conjugated Monoclonal Antibody (Catalog # FAB3195A) or (B) Mouse IgG1Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TRA-1-85/CD147

The TRA-1-85 antigen, also known as OKa blood group antigen, is a specific epitope within the protein known as Basigin, EMMPRIN and CD147. It is a cell surface determinant expressed on almost all human cell types. This antibody has been used in somatic cell hybrid studies to identify tissues of partial human origin (1, 2).

References
  1. Williams, B.P. et al. (1988) Immunogenetics. 27:322.
  2. Spring, F.A. et al. (1997) Eur. J. Immunol. 27:891.
Entrez Gene IDs
682 (Human)
Alternate Names
OKa Blood Group Antigen; TRA185; TRA-1-85

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Citations for Human TRA-1-85/CD147 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. Establishment of a human embryonic stem cell line with homozygous TP53 R248W mutant by TALEN mediated gene editing
    Authors: An Xu, Ruoji Zhou, Jian Tu, Zijun Huo, Dandan Zhu, Donghui Wang et al.
    Stem Cell Research
  2. Generation of human embryonic stem cell line with heterozygous RB1 deletion by CRIPSR/Cas9 nickase
    Authors: Jian Tu, Zijun Huo, Mo Liu, Donghui Wang, An Xu, Ruoji Zhou et al.
    Stem Cell Research
  3. The Hematopoietic Differentiation and Production of Mature Myeloid Cells from Human Pluripotent Stem Cells
    Authors: Kyung-Dal Choi, Maxim Vodyanik, Igor I. Slukvin
    Nature Protocols
  4. Endothelial Cells Promote Expansion of Long-Term Engrafting Marrow Hematopoietic Stem and Progenitor Cells in Primates
    Authors: Jennifer L. Gori, Jason M. Butler, Balvir Kunar, Michael G. Poulos, Michael Ginsberg, Daniel J. Nolan et al.
    Stem Cells Translational Medicine
  5. Patient-derived iPSCs link elevated mitochondrial respiratory complex I function to osteosarcoma in Rothmund-Thomson syndrome
    Authors: BE Jewell, A Xu, D Zhu, MF Huang, L Lu, M Liu, EL Underwood, JH Park, H Fan, JA Gingold, R Zhou, J Tu, Z Huo, Y Liu, W Jin, YH Chen, Y Xu, SH Chen, N Rainusso, NK Berg, DA Bazer, C Vellano, P Jones, HK Eltzschig, Z Zhao, BA Kaipparett, R Zhao, LL Wang, DF Lee
    PloS Genetics, 2021-12-29;17(12):e1009971.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  6. Generation and trapping of a mesoderm biased state of human pluripotency
    Authors: D Stavish, C Böiers, C Price, TJR Frith, J Halliwell, I Saldaña-Gu, J Wray, J Brown, J Carr, C James, I Barbaric, PW Andrews, T Enver
    Nat Commun, 2020-10-05;11(1):4989.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. LGR4, Not LGR5, Enhances hPSC Hematopoiesis by Facilitating Mesoderm Induction via TGF-Beta Signaling Activation
    Authors: Y Wang, H Wang, J Guo, J Gao, M Wang, M Xia, Y Wen, P Su, M Yang, M Liu, L Shi, T Cheng, W Zhou, J Zhou
    Cell Rep, 2020-05-05;31(5):107600.
    Species: Human
    Sample Types: Whole Cells
  8. CD146 is a novel marker for highly tumorigenic cells and a potential therapeutic target in malignant rhabdoid tumor
    Authors: S Nodomi, K Umeda, S Saida, T Kinehara, T Hamabata, T Daifu, I Kato, H Hiramatsu, KI Watanabe, Y Kuwahara, T Iehara, S Adachi, E Konishi, T Nakahata, H Hosoi, T Heike
    Oncogene, 2016-04-04;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. In Vitro Differentiation of First Trimester Human Umbilical Cord Perivascular Cells into Contracting Cardiomyocyte-Like Cells
    Authors: P Szaraz, M Librach, L Maghen, F Iqbal, TA Barretto, S Kenigsberg, A Gauthier-F, CL Librach
    Stem Cells Int, 2016-03-30;2016(0):7513252.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Vascular niche promotes hematopoietic multipotent progenitor formation from pluripotent stem cells.
    Authors: Gori J, Butler J, Chan Y, Chandrasekaran D, Poulos M, Ginsberg M, Nolan D, Elemento O, Wood B, Adair J, Rafii S, Kiem H
    J Clin Invest, 2015-02-09;125(3):1243-54.
    Species: Primate - Macaca nemestrina (Southern Pig-tailed Macaque)
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Sphingosine-1-phosphate promotes expansion of cancer stem cells via S1PR3 by a ligand-independent Notch activation.
    Authors: Hirata, Naoya, Yamada, Shigeru, Shoda, Takuji, Kurihara, Masaaki, Sekino, Yuko, Kanda, Yasunari
    Nat Commun, 2014-09-25;5(0):4806.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Modelling Fanconi anemia pathogenesis and therapeutics using integration-free patient-derived iPSCs.
    Authors: Liu G, Suzuki K, Li M, Qu J, Montserrat N, Tarantino C, Gu Y, Yi F, Xu X, Zhang W, Ruiz S, Plongthongkum N, Zhang K, Masuda S, Nivet E, Tsunekawa Y, Soligalla R, Goebl A, Aizawa E, Kim N, Kim J, Dubova I, Li Y, Ren R, Benner C, del Sol A, Bueren J, Trujillo J, Surralles J, Cappelli E, Dufour C, Esteban C, Izpisua Belmonte J
    Nat Commun, 2014-07-07;5(0):4330.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Induction of pluripotency in human somatic cells via a transient state resembling primitive streak-like mesendoderm.
    Authors: Takahashi K, Tanabe K, Ohnuki M, Narita M, Sasaki A, Yamamoto M, Nakamura M, Sutou K, Osafune K, Yamanaka S
    Nat Commun, 2014-04-24;5(0):3678.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. beta-catenin confers resistance to PI3K and AKT inhibitors and subverts FOXO3a to promote metastasis in colon cancer.
    Authors: Tenbaum SP, Ordonez-Moran P, Puig I, Chicote I, Arques O, Landolfi S, Fernandez Y, Herance JR, Gispert JD, Mendizabal L, Aguilar S, Ramon y Cajal S, Schwartz S, Vivancos A, Espin E, Rojas S, Baselga J, Tabernero J, Munoz A, Palmer HG
    Nat. Med., 2012-06-01;18(6):892-901.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Generation of red blood cells from human induced pluripotent stem cells.
    Authors: Dias J, Gumenyuk M, Kang H, Vodyanik M, Yu J, Thomson JA, Slukvin II
    Stem Cells Dev., 2011-05-11;20(9):1639-47.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Hematopoietic and endothelial differentiation of human induced pluripotent stem cells.
    Authors: Choi KD, Yu J, Smuga-Otto K, Salvagiotto G, Rehrauer W, Vodyanik M, Thomson J, Slukvin I
    Stem Cells, 2009-03-01;27(3):559-67.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Generation of an induced pluripotent stem cell line from an individual with a heterozygous RECQL4 mutation
    Authors: Brittany E. Jewell, Mo Liu, Linchao Lu, Ruoji Zhou, Jian Tu, Dandan Zhu et al.
    Stem Cell Research

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Reviews for Human TRA-1-85/CD147 APC-conjugated Antibody

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Human TRA-1-85/CD147 APC-conjugated Antibody
By Anonymous on 12/15/2016
Application: Flow Sample Tested: induced pluripotent stem cells Species: Human

I use this antibody to quantify total cell numbers in human iPSC induction experiments (to avoid counting feeders cells). I use TRA-1-81 antibody to stain reprogrammed cells and TRA-1-85 antibody to stain total cells, then quantify using FACS, and use the ratio of those two numbers as reprogramming efficiency.

Human TRA-1-85/CD147 APC-conjugated Antibody
By Jingyao Qiu on 08/16/2016
Application: Flow Sample Tested: Cells from hematopoietic differentiation of hESC line H9 Species: Human