Human TMPRSS2 Antibody

Catalog #: MAB10723 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10723-100
MAB10723-SP

Detection of TMPRSS2 in HEK293 Human Cell Line Transfected with Human TMPRSS2 and eGFP by Flow Cytometry

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Human TMPRSS2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TMPRSS2 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 1038127

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human TMPRSS2 synthetic peptide
Accession # O15393

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

HEK293 Human Cell Line Transfected with Human TMPRSS2 and eGFP

Immunocytochemistry

8-25 µg/mL

Immersion fixed LNCaP human prostate cancer cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of TMPRSS2 in HEK293 Human Cell Line Transfected with Human TMPRSS2 and eGFP by Flow Cytometry HEK293 human embryonic kidney cell line transfected with (A) human TMPRSS2 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human TMPRSS2 Monoclonal Antibody (Catalog # MAB10723) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on control antibody staining (MAB0041). Staining was performed using our Staining Membrane-associated Proteins protocol.

Immunocytochemistry

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TMPRSS2 in LNCaP Human Cell Line. TMPRSS2 was detected in immersion fixed LNCaP human prostate cancer cell line (positive staining) and A549 human lung carcinoma cell line (negative staining) using Mouse Anti-Human TMPRSS2 Monoclonal Antibody (Catalog # MAB10723) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TMPRSS2

TMPRSS2 is a transmembrane serine protease. The TMPRSS2 gene is upregulated by androgenic hormones in prostate cells and downregulated in androgen-independent prostate cancer. The fusion between TMPRSS2 gene and ERG is the most common chromosomal aberration detected in prostate cancer. In relation to COVID-19, TMPRSS2 is involved in priming the viral spike protein which facilitates viral entry. TMPRSS2 inhibitors are candidates for treatment options of SARS-CoV-2 and other coronaviruses.

Long Name

Transmembrane protease, serine 2

Entrez Gene IDs

7113 (Human); 50528 (Mouse); 102137447 (Cynomolgus Monkey)

Alternate Names

EC 3.4.21; Epitheliasin; FLJ41954; NP_001128571.1; NP_001369649.1; NP_005647.3; PP9284; PRSS10; Serine protease 10; TMPRSS2; transmembrane protease serine 2; transmembrane protease, serine 2