Human TLR10 Antibody

Catalog #: MAB6619
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6619
MAB6619-SP

Detection of Human TLR10 by Western Blot.

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All TLR10 Antibodies

Product Details

Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TLR10 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TLR10 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) TLR1, 2, 3, 4, 5, 7, 8, 9, recombinant mouse (rm) TLR6, 11, 12, rhIL-1 RI, rhIL-1 RII, rhIL-1 RAcP, rhST2, rhIL-18 R, rhIL-1 Rrp2, rhIL-18 R beta, rhSIGIRR, rhIL-1 RAPL1, rhTIGIRR, rhMD-1, rhMD-2, rhTIRAP, or rmRP105 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 670719

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human TLR10
Asn168-Lys383
Accession # Q9BXR5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human TLR10 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF Membrane was probed with 2 µg/mL of Human TLR10 Monoclonal Antibody (Catalog # MAB6619) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TLR10 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TLR10

Toll-like receptor 10 (TLR10, CD290) is a 91-100 kDa member of the Toll-like receptor family. Human TLR10 is synthesized as a precursor with a 19 amino acid (aa) signal sequence and a 792 aa mature chain. The mature chain constitutes a single-pass type I transmembrane protein. The extracellular domain (ECD) extends from aa 20-576 and the cytoplasmic region consists of aa 598-811. Human TLR10 contains 15 leucine-rich repeats plus eight potential sites for N-linked glycosylation. In addition, it contains one cytoplasmic TIR domain. Functionally, TLR10 is involved in the innate immune response to microbial agents. Specifically, it acts via MYD88 and TRAF6, which leads to NF-kappa-B activation, cytokine secretion, and an inflammatory response. Human TLR10 shares 71% aa sequence identity with rat TLR10.

Long Name

Toll-like Receptor 10

Entrez Gene IDs

81793 (Human)

Alternate Names

CD290 antigen; CD290; MGC104967; MGC126398; MGC126399; TLR10; toll-like receptor 10

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human TLR10 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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