Human Testican 1/SPOCK1 Antibody

Catalog #: MAB2327
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2327
MAB2327-SP

Product Details

Citations (2)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Testican 1/SPOCK1 Antibody Summary

Specificity

Detects human Testican 1/SPOCK1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Testican 2 or rhTestican 3 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 316801

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Testican 1/SPOCK1
Arg22-Trp439
Accession # Q08629

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Testican 1/SPOCK1 (Catalog # 2327-PI)

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Testican 1/SPOCK1 (Catalog # 2327-PI), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Testican 1/SPOCK1

Testican 1, also known as SPOCK1, was originally identified in seminal plasma and is also expressed in brain. It is a proteoglycan that contains a Ca2+-binding domain and a C-terminal acidic domain with putative glycosaminoglycan attachment sites as well as three potential inhibitory domains targeted toward three different classes of proteases, metallo-, cysteine and serine proteases. Human Testican 1 shares 95% amino acid sequence identity with mouse Testican 1.

Entrez Gene IDs

6695 (Human)

Alternate Names

FLJ37170; Protein SPOCK; sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 1; SPOCK1; SPOCKcwcv and kazal-like domains proteoglycan (testican); Testican 1; TIC1; TICN1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Testican 1/SPOCK1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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SPOCK1 is a novel inducer of epithelial to mesenchymal transition in drug-induced gingival overgrowth
Authors: R Alshargabi, T Sano, A Yamashita, A Takano, T Sanada, M Iwashita, T Shinjo, T Fukuda, T Sanui, S Kishida, F Nishimura
Sci Rep, 2020-06-17;10(1):9785.
Species: Human, Mouse
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC, Western Blot
Broad regulation of matrix and adhesion molecules in THP-1 human macrophages by nitroglycerin.
Authors: Krishnatry AS, Brazeau DA, Fung HL
Nitric Oxide, 2009-10-15;22(1):11-7.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot