Human TAFA4/FAM19A4 Antibody

Catalog #: MAB5099 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5099
MAB5099-SP

Detection of TAFA4/FAM19A4 in A172 Human Cell Line by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TAFA4/FAM19A4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TAFA4/FAM19A4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human TAFA1, 2, 3, or 5 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 480103

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human TAFA4
Ser35-Arg140
Accession # Q96LR4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human TAFA4/FAM19A4 (Catalog # 5099-TA) under non-reducing conditions only

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of TAFA4/FAM19A4 antibody in A172 Human Cell Line antibody by Flow Cytometry.

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Detection of TAFA4/FAM19A4 in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Mouse Anti-Human TAFA4/FAM19A4 Monoclonal Antibody (Catalog # MAB5099, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TAFA4/FAM19A4

TAFA4 (also FAM19A4) is a secreted, 12 kDa member of the FAM19/TAFA family of chemokine-like proteins (1). It is synthesized as a 140 amino acid (aa) precursor that contains a 35 aa signal sequence and a 105 aa mature chain. Like other members of the FAM19/TAFA family, with the exception of TAFA5, mature TAFA1 contains 10 regularly spaced cysteine residues that follow the pattern Cx₇CCx₁₃CxCx₁₄Cx₁₁Cx₄Cx₅Cx₁₀C, where C represents a conserved cysteine residue and x represents any noncysteine amino acid (1). Human TAFA4 is 90% aa identical to mouse TAFA4 (1). Real-time PCR analysis indicates that TAFA4 mRNA expression is restricted to the central nervous system (CNS), with the highest level in the thalamus (1). TAFAs may modulate immune responses in the CNS by functioning as brain specific chemokines and may act with other chemokines to optimize the recruitment and activity of immune cells in the CNS (1). TAFAs may represent a novel class of neurokines that act as regulators of immune nervous cells (1, 2). TAFAs may also control axonal sprouting following brain injury (1).

References