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Human Synaptophysin Antibody

Catalog #: AF5555
11 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF5555
AF5555-SP
Synaptophysin in Human Brainstem Tissue.
3 Images
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Citations (11)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Synaptophysin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Synaptophysin in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Synaptophysin
Glu50-Phe106
Accession # P08247
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
50 µg/mL
Human cerebellum
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Synaptophysin antibody in Human Brainstem Tissue by Immunohistochemistry (IHC-P). View Larger

Synaptophysin in Human Brainstem Tissue. Synaptophysin was detected in immersion fixed paraffin-embedded sections of human brainstem tissue using Goat Anti-Human Synaptophysin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5555) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to olivary nucleus. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. *Not recommended for Immunohistochemistry/Immunocytochemistry on mouse and rat samples.

Western Blot Detection of Human, Mouse, and Rat Synaptophysin antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat Synaptophysin by Western Blot. Western blot shows lysates of human brain tissue, mouse brain tissue, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Synaptophysin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5555) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Synaptophysin at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western View Larger

Detection of Human Synaptophysin by Simple WesternTM. Simple Western shows lysates of human cerebellum, loaded at 0.5 mg/ml. A specific band was detected for Synaptophysin at approximately 59 kDa (as indicated) using 50 µg/mL of Goat Anti-Human Synaptophysin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5555). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Synaptophysin

Synaptophysin (also p38 and BM89 in mouse) is a 38‑40 kDa member of the synaptophysin/synaptobrevin family of proteins. It is expressed in neurons, and interacts with VAMP2 at an early stage of vesicle formation, guiding VAMP2 placement into secretory vesicles that are destined for release upon stimulation. Human Synaptophysin is a four transmembrane protein that is 313 amino acids (aa) in length. It contains two luminal domains (aa 50‑106 and 162‑199) and a cytoplasmic C‑terminal targeting region that specifies its localization (aa 214‑313). Synaptophysin forms homodimers and oligomers, and heterodimerizes with VAMP2. There is one isoform variant that shows a six aa substitution for aa 35‑313. Over aa 50‑106, human Synaptophysin shares 89% aa identity with mouse Synaptophysin.

Entrez Gene IDs
6855 (Human)
Alternate Names
Major synaptic vesicle protein p38; MRX; MRXSYP; Synaptophysin; SYP

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Citations for Human Synaptophysin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. TMEM106B deficiency impairs cerebellar myelination and synaptic integrity with Purkinje cell loss
    Authors: T Feng, L Luan, II Katz, M Ullah, VM Van Deerli, JQ Trojanowsk, EB Lee, F Hu
    Acta neuropathologica communications, 2022-03-14;10(1):33.
  2. Mesenchymal properties of iPSC-derived neural progenitors that generate undesired grafts after transplantation
    Authors: Miho Isoda, Tsukasa Sanosaka, Ryo Tomooka, Yo Mabuchi, Munehisa Shinozaki, Tomoko Andoh-Noda et al.
    Communications Biology
  3. Concerted type I interferon signaling in microglia and neural cells promotes memory impairment associated with amyloid ? plaques
    Authors: Roy ER, Chiu G, Li S et al.
    Immunity
  4. Epithelial-derived factors induce muscularis mucosa of human induced pluripotent stem cell-derived gastric organoids
    Authors: K Uehara, M Koyanagi-A, T Koide, T Itoh, T Aoi
    Stem Cell Reports, 2022-03-03;0(0):.
    Species: Mouse
    Sample Types: Tissue Lysates
    Applications: Western Blot
  5. Retina Organoid Transplants Develop Photoreceptors and Improve Visual Function in RCS Rats With RPE Dysfunction
    Authors: Bin Lin, Bryce T. McLelland, Robert B. Aramant, Biju B. Thomas, Gabriel Nistor, Hans S. Keirstead et al.
    Investigative Opthalmology & Visual Science
  6. Human iPSC-Derived Blood-Brain Barrier Chips Enable Disease Modeling and Personalized Medicine Applications
    Authors: GD Vatine, R Barrile, MJ Workman, S Sances, BK Barriga, M Rahnama, S Barthakur, M Kasendra, C Lucchesi, J Kerns, N Wen, WR Spivia, Z Chen, J Van Eyk, CN Svendsen
    Cell Stem Cell, 2019-06-06;24(6):995-1005.e6.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. Cerebral organoids at the air-liquid interface generate diverse nerve tracts with functional output
    Authors: SL Giandomeni, SB Mierau, GM Gibbons, LMD Wenger, L Masullo, T Sit, M Sutcliffe, J Boulanger, M Tripodi, E Derivery, O Paulsen, A Lakatos, MA Lancaster
    Nat. Neurosci., 2019-03-18;22(4):669-679.
    Species: Human
    Sample Types: Organoid
    Applications: IHC
  8. Neurotransmitter Diversity In Pre-Synaptic Terminals Located in the Parvicellular Neuroendocrine Paraventricular Nucleus of the Rat and Mouse Hypothalamus
    Authors: CS Johnson, JS Bains, AG Watts
    J. Comp. Neurol., 2018-03-02;0(0):.
    Species: Mouse, Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  9. The Proinflammatory Cytokine High-Mobility Group Box-1 Mediates Retinal Neuropathy Induced by Diabetes
    Authors: Ahmed M. Abu El-Asrar, Mohammad Mairaj Mairaj Siddiquei, Mohd Imtiaz Nawaz, Karel Geboes, Ghulam Mohammad
    Mediators of Inflammation
  10. Axonal BACE1 dynamics and targeting in hippocampal neurons: a role for Rab11 GTPase.
    Authors: Buggia-Prevot V, Fernandez C, Riordan S, Vetrivel K, Roseman J, Waters J, Bindokas V, Vassar R, Thinakaran G
    Mol Neurodegener, 2014-01-04;9(0):1.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. The Alzheimer’s beta -secretase BACE1 localizes to normal presynaptic terminals and to dystrophic presynaptic terminals surrounding amyloid plaques
    Authors: Patty C. Kandalepas, Katherine R. Sadleir, William A. Eimer, Jie Zhao, Daniel A. Nicholson, Robert Vassar
    Acta Neuropathologica

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