Human Sulfatase Modifying Factor 2/SUMF2 Antibody

Catalog # Availability Size / Price Qty
MAB3594
MAB3594-SP
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Human Sulfatase Modifying Factor 2/SUMF2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Sulfatase Modifying Factor 2/SUMF2 in direct ELISAs and Western blots. No cross-reactivity with recombinant human SUMF1 or recombinant mouse SUMF2 is observed.
Source
Monoclonal Mouse IgG2B Clone # 330312
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Sulfatase Modifying Factor 2/SUMF2
Gln26-Leu301
Accession # Q8NBJ7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Sulfatase Modifying Factor 2/SUMF2
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human
Sulfatase Modifying Factor 2/SUMF2, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Sulfatase Modifying Factor 2/SUMF2

Also known as the paralog of C‑ alpha ‑formylglycine-generating enzyme/SUMF1, SUMF2 may compete with SUMF1 for the same sulfatase, but it does not act as C‑ alpha ‑formylglycine-generating enzyme. The amino acid sequence of recombinant human SUMF2 is 90%, 88%, 86% and 85% identical to that of bovine, canine, rat, and mouse.

Entrez Gene IDs
25870 (Human)
Alternate Names
C-alpha-formyglycine-generating enzyme 2; C-alpha-formylglycine-generating enzyme 2; DKFZp566I1024; DKFZp686I1024; DKFZp686L17160; DKFZp781L1035; FGE2; MGC99485; paralog of the formylglycine-generating enzyme; pFGE; sulfatase modifying factor 2; sulfatase-modifying factor 2; SUMF2

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