Human Sulfatase Modifying Factor 1/SUMF1 Antibody

Catalog #: AF3680
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3680
AF3680-SP

Detection of Human Sulfatase Modifying Factor 1/SUMF1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Sulfatase Modifying Factor 1/SUMF1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human SUMF1 in direct ELISAs and Western blots. In these formats, approximately 45% cross-reactivity with recombinant mouse SUMF1 is observed and less than 1% cross-reactivity with recombinant human SUMF2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human SUMF1 isoform 1
Ser34-Asp374 with a Ser63Asn substitution
Accession # Q8NBK3

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Sulfatase Modifying Factor 1/SUMF1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, MCF-7 human breast cancer cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Sulfatase Modifying Factor 1/SUMF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3680) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for Sulfatase Modifying Factor 1/SUMF1 at approximately 40 and 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Sulfatase Modifying Factor 1/SUMF1

Human SUMF-1 (also FGE) is a 42 kDa (341 aa), Ca⁺⁺-binding member of the sulfatase-modifying factor family. It is a soluble ER lumenal glycoprotein that converts inactive sulfatases into an active form by transforming a catalytic site cysteine into a formylglycine residue. In the ER, SUMF-1 can exist as either a monomer, or a disulfide-linked homodimer or a heterodimer with SUMF-2. Three splice isoforms are known. Mature human SUMF-1 shares 90% aa sequence identity with mouse SUMF-1.

Entrez Gene IDs

285362 (Human); 58911 (Mouse); 362409 (Rat)

Alternate Names

AAPA3037; EC 1.8.99.-; FGE1; FGEC-alpha-formylglycine-generating enzyme 1; FGly-generating enzyme; MGC131853; MGC150436; sulfatase modifying factor 1; sulfatase-modifying factor 1; SUMF1; UNQ3037

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citation for Human Sulfatase Modifying Factor 1/SUMF1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Drug screening identifies tazarotene and bexarotene as therapeutic agents in multiple sulfatase deficiency
Authors: L Schlotawa, K Tyka, M Kettwig, RC Ahrens-Nic, M Baud, T Berulava, N Brunetti-P, A Gagne, ZM Herbst, JA Maguire, J Monfregula, T Pena, K Radhakrish, S Schröder, EA Waxman, A Ballabio, T Dierks, A Fischer, DL French, MH Gelb, J Gärtner
Embo Molecular Medicine, 2023-02-15;0(0):e14837.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot