Human ST2/IL-33R Antibody

Catalog #: MAB5233 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB5233-100
MAB5233-SP

Detection of Human ST2/IL-33 R by Western Blot.

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All ST2/IL-33R Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ST2/IL-33R Antibody Summary

Species Reactivity

Human

Specificity

Detects human ST-2/IL-33R in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2154D

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human ST-2/IL-33 R
Lys19-Phe328
Accession # Q01638

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human ST2/IL-33 R by Western Blot. Western blot shows lysates of human kidney tissue and small intestine tissue. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human ST2/IL-33 R Monoclonal Antibody (Catalog # MAB5233) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for ST2/IL-33 R at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ST2/IL-33R

ST2, also known as IL-33R, IL-1 R4 and T1, is an Interleukin-1 receptor family glycoprotein that contributes to Th2 immune responses (1, 2). Human ST2 consists of a 310 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 207 aa cytoplasmic domain with an intracellular TIR domain (3, 4). Alternate splicing of the 120 kDa human ST2 generates a soluble 60 kDa isoform that lacks the transmembrane and cytoplasmic regions as well as an isoform that additionally lacks the third Ig‑like domain (4). Within the ECD, human ST2 shares 68% and 64% aa sequence identity with mouse and rat ST2, respectively. ST2 is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes (5‑8). It binds IL-33, a cytokine that is upregulated by inflammation or mechanical strain in smooth muscle cells, airway epithelia, keratinocytes, and cardiac fibroblasts (5, 9). IL-33 binding induces the association of ST2 with IL-1R AcP, a shared signaling subunit that also associates with IL-1 RI and IL-1 R rp2 (1, 10, 11). In macrophages, ST2 interferes with signaling from IL-1 RI and TLR4 by sequestering the adaptor proteins MyD88 and Mal (7). In addition to its role in promoting mast cell and Th2 dependent inflammation, ST2 activation enhances antigen induced hypernociception and protects from atherosclerosis and cardiac hypertrophy (5, 12‑14). The soluble ST2 isoform is released by activated Th2 cells and strained cardiac myocytes and is elevated in the serum in allergic asthma (6, 8, 15). Soluble ST2 functions as a decoy receptor that blocks IL‑33's ability to signal through transmembrane ST2 (10, 13‑15).

References

Barksby, H.E. et al. (2007) Clin. Exp. Immunol. 149:217.
Gadina, M. and C.A. Jefferies (2007) Science STKE 2007:pe31.
Tominaga, S. et al. (1992) Biochim. Biophys. Acta 1171:215.
Li, H. et al. (2000) Genomics 67:284.
Schmitz, J. et al. (2005) Immunity 23:479.
Lecart, S. et al. (2002) Eur. J. Immunol. 32:2979.
Brint, E.K. et al. (2004) Nat. Immunol. 5:373.
Weinberg, E.O. et al. (2002) Circulation 106:2961.
Sanada S. et al. (2007) J. Clin. Invest. 117:1538.
Palmer, G. et al. (2008) Cytokine 42:358.
Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
Allakhverdi, Z. et al. (2007) J. Immunol. 179:2051.
Verri Jr., W.A. et al. (2008) Proc. Natl. Acad. Sci. 105:2723.
Miller, A.M. et al. (2008) J. Exp. Med. 205:339.
Hayakawa, H. et al. (2007) J. Biol. Chem. 282:26369.

Long Name

Interleukin 33 Receptor

Entrez Gene IDs

9173 (Human); 17082 (Mouse)

Alternate Names

DER4; DER4ST2growth stimulation-expressed; Fit-1; IL-1 R4; IL1R4; IL-1R4; IL-1RL1; IL-33 R; IL33R; IL-33R; interleukin 1 receptor-like 1; interleukin 1 receptor-related protein; interleukin-1 receptor-like 1; Ly84; MGC32623; Protein ST2; ST2; ST2L; ST2V; T1; T1homolog of mouse growth stimulation-expressed