Human SRPK2 Antibody

Catalog #: AF5530 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5530
AF5530-SP

Detection of Human SRPK2 by Western Blot.

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Human SRPK2 Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human SRPK2 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human SRPK2
Asn313-Ala466
Accession # P78362

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human SRPK2 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, U937 human histiocytic lymphoma cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Human SRPK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5530) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SRPK2 at approximately 115 to 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SRPK2

SRPK2 (Ser/Arg-rich protein specific kinase 2) is a cytoplasmic, 115‑120 kDa member of the CMGC Ser/Thr protein kinase family of enzymes. It is expressed in many experimental cell lines and serves to selectively phosphorylate Ser on RS domain-containing proteins. This phosphorylation activates RS domain-containing non-snRNP proteins in the spliceosome complex, and initiates mRNA splicing. Human SRPK2 is 688 amino acids (aa) in length. It contains a Pro-rich region (aa 21‑45) that may interact with WW domain-containing proteins, and a split kinase domain (aa 79‑226 and 524‑646). Phosphorylation on S52 and S588 regulates activity. There are at least two splice variants. One shows a 24 aa substitution for aa 1‑13, while a second shows a nine aa substitution for aa 538‑688.

Long Name

SFRS Protein Kinase 2

Entrez Gene IDs

6733 (Human); 20817 (Mouse); 296753 (Rat)

Alternate Names

EC 2.7.11.1; FLJ36101; H_RG152G17.1a; H_RG152G17.1b; serine kinase SRPK2; Serine/arginine-rich protein-specific kinase 2; serine/arginine-rich splicing factor kinase 2; serine/threonine-protein kinase SRPK2; SFRS protein kinase 2SFRSK2; SFRSK2; SR protein kinase 2; SRPK2; SR-protein-specific kinase 2; SRSF protein kinase 2; WUGSC:H_RG152G17.1a