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Human SOX3 Antibody

Catalog #: AF2569
13 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2569
AF2569-SP
SOX3 in NTera‑2 Human Cell Line.
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Product Details
Citations (13)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SOX3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human SOX3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) SOX2, rhSOX7, rhSOX10, and rhSOX17 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human SOX3
Met1-Leu230
Accession # P41225
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human SOX3
Immunocytochemistry
5-15 µg/mL
Immersion fixed NTera-2 human testicular embryonic carcinoma cell line, U‑251 MG human glioblastoma cells (positive) and HDLM‑2 human Hodgkin's lymphoma cells (negative)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry SOX3 antibody in NTera-2 Human Cell Line by Immunocytochemistry (ICC). View Larger

SOX3 in NTera‑2 Human Cell Line. SOX3 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line differentiation with retinoic acid using Goat Anti-Human SOX3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2569) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green, upper panel; NL003) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry View Larger

Detection of SOX3 in U‑251 MG cells (positive) and HDLM‑2 cells (negative). SOX3 was detected in immersion fixed U‑251 MG human glioblastoma cells (positive) and absent in HDLM‑2 human Hodgkin’s lymphoma cells (negative) using Goat Anti-Human SOX3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2569) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOX3

SOX3 belongs to the SOX family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The SOX3 gene is localized on the X chromosome and is expressed in the brain and gonads. It has been suggested that SOX3 plays a role in brain formation and function. Human and mouse SOX3 share 92% amino acid sequence identity.

Long Name
Transcription Factor SOX3
Entrez Gene IDs
6658 (Human)
Alternate Names
GHDX; MRGH; panhypopituitarism; PHP; PHPX; SOX3; SOXB; SRY (sex determining region Y)-box 3; transcription factor SOX-3

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Citations for Human SOX3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Spermatogonial fate in mice with increased activin A bioactivity and testicular somatic cell tumours
    Authors: Penny A. F. Whiley, Benedict Nathaniel, Peter G. Stanton, Robin M. Hobbs, Kate L. Loveland
    Frontiers in Cell and Developmental Biology
  2. Chromatin remodelers HELLS, WDHD1 and BAZ1A are dynamically expressed during mouse spermatogenesis
    Authors: Ram Prakash Yadav, Sini Leskinen, Lin Ma, Juho-Antti Mäkelä, Noora Kotaja
    Reproduction
  3. Single-cell transcriptomic landscapes of the otic neuronal lineage at multiple early embryonic ages
    Authors: Y Sun, L Wang, T Zhu, B Wu, G Wang, Z Luo, C Li, W Wei, Z Liu
    Cell Reports, 2022-03-22;38(12):110542.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  4. The Nestin neural enhancer is essential for normal levels of endogenous Nestin in neuroprogenitors but is not required for embryo development
    Authors: E Thomson, R Dawson, CH H'ng, F Adikusuma, S Piltz, PQ Thomas
    PLoS ONE, 2021-11-05;16(11):e0258538.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. An mTORC1-dependent switch orchestrates the transition between mouse spermatogonial stem cells and clones of progenitor spermatogonia
    Authors: S Suzuki, JR McCarrey, BP Hermann
    Cell Reports, 2021-02-16;34(7):108752.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  6. SOX3 promotes generation of committed spermatogonia in postnatal mouse testes
    Authors: D McAninch, JA Mäkelä, HM La, JN Hughes, R Lovell-Bad, RM Hobbs, PQ Thomas
    Sci Rep, 2020-04-21;10(1):6751.
    Species: Mouse
    Sample Types: Tissue Homogenate
    Applications: Immunoprecipitation
  7. DDX5 plays essential transcriptional and post-transcriptional roles in the maintenance and function of spermatogonia
    Authors: JMD Legrand, AL Chan, HM La, FJ Rossello, ML Änkö, FV Fuller-Pac, RM Hobbs
    Nat Commun, 2019;10(1):2278.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  8. Identification of dynamic undifferentiated cell states within the male germline
    Authors: HM La, JA Mäkelä, AL Chan, FJ Rossello, CM Nefzger, JMD Legrand, M De Seram, JM Polo, RM Hobbs
    Nat Commun, 2018-07-19;9(1):2819.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. GILZ-dependent modulation of mTORC1 regulates spermatogonial maintenance
    Authors: Hue M. La, Ai-Leen Chan, Julien M. D. Legrand, Fernando J. Rossello, Christina G. Gangemi, Antonella Papa et al.
    Development
  10. An Eya1-Notch axis specifies bipotential epibranchial differentiation in mammalian craniofacial morphogenesis
    Authors: Haoran Zhang, Li Wang, Elaine Yee Man Wong, Sze Lan Tsang, Pin-Xian Xu, Urban Lendahl et al.
    eLife
  11. Identification of Highly Conserved Putative Developmental Enhancers Bound by SOX3 in Neural Progenitors Using ChIP-Seq
    Authors: Dale McAninch, Paul Thomas
    PLoS ONE
  12. Congenital Hydrocephalus and Abnormal Subcommissural Organ Development in Sox3 Transgenic Mice
    Authors: Kristie Lee, Jacqueline Tan, Michael B. Morris, Karine Rizzoti, James Hughes, Pike See Cheah et al.
    PLoS ONE
  13. Identification of SOX3 as an XX male sex reversal gene in mice and humans.
    Authors: Sutton E, Hughes J, White S
    J. Clin. Invest., 2010-12-22;121(1):328-41.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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