Human SOX18 Antibody

Catalog #: AF5077 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5077
AF5077-SP

Detection of Human SOX18 by Western Blot.

1 Image

All SOX18 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SOX18 Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human SOX18 in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human SOX18
Ala276-Gly384
Accession # P35713

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human SOX18 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HUVEC human umbilical vein endothelial cells, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human SOX18 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5077) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SOX18 at approximately 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOX18

SOX18 (SRY-related HMG box) is a 41 kDa (predicted) Group F member of the SOX family of DNA-binding proteins. Human SOX18 is 384 amino acids (aa) in length and contains an N-terminal DNA-binding HMG box (aa 85‑153), a central TAD region (aa 168‑260) and a highly charged sequence that contains a nine aa transactivation motif (aa 323‑331). SOX18 is expressed by endothelium and vascular smooth muscle, and apparently promotes the formation and organization of new capillaries. Over aa 276‑384, human SOX18 shares 90% and 89% aa identity with mouse and porcine SOX18, respectively.

Long Name

SRY-related HMG-box 18

Entrez Gene IDs

54345 (Human); 20672 (Mouse); 311723 (Rat)

Alternate Names

HLTS; SOX18; SRY (sex determining region Y)-box 18; SRY-box 18; transcription factor SOX-18