Human SMAGP Antibody

Catalog #: AF3959 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3959
AF3959-SP

Detection of Human SMAGP by Western Blot.

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Human SMAGP Antibody Summary

Species Reactivity

Human

Specificity

Detects human SMAGP in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross-reactivity with recombinant rat SMAGP is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human SMAGP
Thr2-Thr34, Phe56-Ile97
Accession # Q0VAQ4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human SMAGP by Western Blot. Western blot shows lysates of human lung tissue, PC-3 human prostate cancer cell line, HT-29 human colon adenocarcinoma cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SMAGP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3959) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for SMAGP at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry

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SMAGP in Human Colon. SMAGP was detected in immersion fixed paraffin-embedded sections of human colon using 15 µg/mL Goat Anti-Human SMAGP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3959) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SMAGP

SMAGP (small transmembrane and glycosylated protein) is a 25 kDa member of the SMAGP family of proteins. It is expressed on breast, colon and biliary duct epithelium, and likely plays a role in cell-cell adhesion. Human SMAGP is an O-glycosylated, 97 amino acid (aa) type III transmembrane glycoprotein that contains a signal sequenceless N-terminal extracellular region (aa 1-34) plus a 39 aa C-terminal cytoplasmic domain. Over the intra- and extracellular regions combined, human SMAGP is 76% aa identical to mouse SMAGP.

Long Name

Small Transmembrane and Glycosylated Protein

Entrez Gene IDs

57228 (Human); 207818 (Mouse); 300236 (Rat)

Alternate Names

hSMAGP; LOC57228; MGC149453; MGC149454; SMAGP; small cell adhesion glycoprotein; small cell transmembrane and glycosylated protein; Small transmembrane and glycosylated protein; small trans-membrane and glycosylated protein