Human Smad4 Antibody Summary
Pro139-Asp332
Accession # Q13485
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Smad4 in HeLa Human Cell Line. Smad4 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Smad4 Monoclonal Antibody (Catalog # MAB2097) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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Detection of Smad4 in HeLa Human Cell Line by Flow Cytometry. HeLa human cell line was stained with Mouse Anti-Human Smad4 Monoclonal Antibody (Catalog # MAB2097, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Smad4
Mothers Against Decapentaplegic homolog 4 (Smad4) belongs to a family of intracellular proteins that transmit transforming growth factor beta (TGF-beta ) superfamily signals from the cell surface to the nucleus. Upon signal-induced phosphorylation, Smad subunits associate with the common-mediator subunit, Smad4. This heteromeric complex then translocates into the nucleus to exert transcriptional comodulator activity.
Product Datasheets
Product Specific Notices
This product is covered by one or more of the following US patents: 5,712,097; 5,814,457; 5,955,292 and other pending US patent applications and foreign equivalents.Citation for Human Smad4 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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PAI-1-Dependent Inactivation of SMAD4-Modulated Junction and Adhesion Complex in Obese Endometrial Cancer
Authors: LL Lin, ER Kost, CL Lin, P Valente, CM Wang, MG Kolonin, AC Daquinag, X Tan, N Lucio, CN Hung, CP Wang, NB Kirma, TH Huang
Cell Rep, 2020-10-13;33(2):108253.
Species: Human
Sample Types: Whole Cells
Applications: CyTof
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10^6 MCF-7 cells were incubated with 0.50 ug of Mouse anti-Human Smad4 antibody (red), or Mouse Isotype Control IgG (black), followed by PE-conjugated anti-Mouse secondary antibody.
