Human Siglec-9 APC-conjugated Antibody

Catalog #: FAB1139A
7 Citations Datasheet / COA / SDS

Clone 191240 was used by HLDA to establish CD designation

Catalog #	Availability	Size / Price	Qty
FAB1139A-025
FAB1139A-100

Detection of Siglec‑9 in Human Blood Granulocytes by Flow Cytometry.

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Citations (7)

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Human Siglec-9 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Siglec-9 in ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Siglec-2, -3, -5, -7 or -10 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 191240

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Siglec-9
Gln18-Gly348 (predicted)
Accession # Q9Y336

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Siglec‑9 in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with (A) Mouse Anti-Human Siglec-3/CD33 PE-conjugated Monoclonal Antibody (Catalog # FAB1137P), and (B) Mouse Anti-Human Siglec-9 APC-conjugated Monoclonal Antibody (Catalog # FAB1139A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Siglec-9

Siglecs(1) (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains (1, 2). Eleven human Siglecs have been cloned and characterized. They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglec-5 to -11 (1‑4). To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. Siglecs 5 to 11 share a high degree of sequence similarity with CD33/Siglec-3 both in their extracellular and intracellular regions. They are collectively referred to as CD33-related Siglecs. One remarkable feature of the CD33-related Siglecs is their differential expression pattern within the hematopoietic system (2, 3). This fact, together with the presence of two conserved immunoreceptor tyrosine-based inhibition motifs (ITIMs) in their cytoplasmic tails, suggests that CD33-related Siglecs are involved in the regulation of cellular activation within the immune system.

The cDNA of human Siglec-9 encodes a 463 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-likeV-type domain, two Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail (5, 6). In peripheral blood leukocytes, Siglec-9 is expressed on neutrophils, monocytes, a fraction of NK cells, B cells, and a minor subset of CD8+ T cells (5). It binds equally well to both 2,3- and 2,6-linked sialic acid (5, 6). Siglec-9 is closely related to Siglec-7, and they share ~80% amino acid sequence identity. The gene encoding siglec-9 was mapped to chromosome 19q13.4.

References

Crocker, P.R. et al. (1998) Glycobiology 8:v.
Crocker, P.R. and A. Varki (2001) Trends Immunol. 22:337.
Crocker, P.R. and A. Varki (2001) Immunology 103:137.
Angata, T. et al. (2002) J. Biol. Chem. 277:24466.
Zhang, J.Q. et al. (2000) J. Biol. Chem. 275:22121.
Angata, T. et al. (2000) J. Biol. Chem. 275:22127.

Long Name

Sialic Acid Binding Ig-like Lectin 9

Entrez Gene IDs

27180 (Human); 102118310 (Cynomolgus Monkey)

Alternate Names

CD329; CDw329; FOAP-9; OBBP-Like; Protein FOAP-9; sialic acid binding Ig-like lectin 9; sialic acid-binding Ig-like lectin 9; Siglec9; Siglec-9

Product Datasheets

Product Datasheet

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Related Research Areas

Citations for Human Siglec-9 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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The lytic polysaccharide monooxygenase CbpD promotes Pseudomonas aeruginosa virulence in systemic infection
Authors: F Askarian, S Uchiyama, H Masson, HV Sørensen, O Golten, AC Bunæs, S Mekasha, ÅK Røhr, E Kommedal, JA Ludviksen, MØ Arntzen, B Schmidt, RH Zurich, NM van Sorge, VGH Eijsink, U Krengel, TE Mollnes, NE Lewis, V Nizet, G Vaaje-Kols
Nature Communications, 2021-02-23;12(1):1230.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Identification of HIV transmitting CD11c+ human epidermal dendritic cells
Authors: KM Bertram, RA Botting, H Baharlou, JW Rhodes, H Rana, JD Graham, E Patrick, J Fletcher, TM Plasto, NR Truong, C Royle, CM Doyle, O Tong, N Nasr, L Barnouti, MP Kohout, AJ Brooks, MP Wines, P Haertsch, J Lim, MP Gosselink, G Ctercteko, JD Estes, MJ Churchill, PU Cameron, E Hunter, MA Haniffa, AL Cunningham, AN Harman
Nat Commun, 2019-06-21;10(1):2759.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Siglec-9 regulates an effector memory CD8+ T-cell subset that congregates in the melanoma tumor microenvironment
Authors: Q Haas, K Frias Boli, C Jandus, C Schneider, C Simillion, MA Stanczak, M Haubitz, SM Seyed Jafa, A Zippelius, GM Baerlocher, H Läubli, RE Hunger, P Romero, HU Simon, S von Gunten
Cancer Immunol Res, 2019-04-15;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Phenotypic and functional consequences of different isolation protocols on skin mononuclear phagocytes
Authors: RA Botting, KM Bertram, H Baharlou, KJ Sandgren, J Fletcher, JW Rhodes, H Rana, TM Plasto, XM Wang, JJ Lim, L Barnouti, MP Kohout, T Papadopoul, S Merten, N Olbourne, AL Cunningham, M Haniffa, AN Harman
J. Leukoc. Biol, 2017-03-07;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Staphylococcus aureus Staphopain A inhibits CXCR2-dependent neutrophil activation and chemotaxis.
EMBO J., 2012-07-31;31(17):3607-19.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
CMV drives clonal expansion of NKG2C+ NK cells expressing self-specific KIRs in chronic hepatitis patients.
Authors: Beziat V, Dalgard O, Asselah T, Halfon P, Bedossa P, Boudifa A, Hervier B, Theodorou I, Martinot M, Debre P, Bjorkstrom NK, Malmberg KJ, Marcellin P, Vieillard V
Eur. J. Immunol., 2011-12-16;42(2):447-57.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Expression profiles of novel cell surface molecules on B-cell subsets and plasma cells as analyzed by flow cytometry.
Authors: Llinas L, Lazaro A, de Salort J, Matesanz-Isabel J, Sintes J, Engel P
Immunol. Lett., 2010-10-15;134(2):113-21.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry