Human Siglec-5/Siglec-14 Biotinylated Antibody Summary
Lys18-Thr434
Accession # O15389
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![Detection of Siglec-5 and Siglec-14 antibody in Human Monocytes and Granulocytes antibody by Flow Cytometry. Detection of Siglec-5 and Siglec-14 antibody in Human Monocytes and Granulocytes antibody by Flow Cytometry.](https://aeroglobalimagesuat.blob.core.windows.net/images/datasheets/antibody/Siglec-5_Siglec-14_BAF1072_Flow_Cytometry_8285.jpg)
Detection of Siglec‑5 and Siglec-14 in Human Monocytes and Granulocytes by Flow Cytometry. Human whole blood monocytes and granulocytes were stained with Human Siglec‑5/14 Biotinylated Antigen Affinity‑purified Polyclonal Antibody (Catalog # BAF1072, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Siglec-5/Siglec-14
Siglecs (1) (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins (2) belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding (3), followed by varying numbers of Ig-like C2-type domains (1, 4). Eleven human Siglecs have been cloned and characterized (1, 4). They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and the Siglec-5 to 11 (4, 5, 6). To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. Siglec-5 to 11 share a high degree of sequence similarity with CD33/Siglec-3 both in their extracellular and intracellular regions. They are collectively referred to as CD33-related Siglecs. One remarkable feature of the CD33-related Siglecs is their differential expression pattern within the hematopoietic system (4, 5). This fact, together with the presence of two conserved immunoreceptor tyrosine-based inhibition motifs (ITIMs) in their cytoplasma tails, suggests that CD33-related Siglecs are involved in the regulation of cellular activation within the immune system.
Product Datasheets
Citations for Human Siglec-5/Siglec-14 Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Soluble Siglec-5 associates to PSGL-1 and displays anti-inflammatory activity
Sci Rep, 2016-11-28;6(0):37953.
Applications: ELISA Development (Detection) -
Siglecs facilitate HIV-1 infection of macrophages through adhesion with viral sialic acids.
Authors: Zou Z, Chastain A, Moir S
PLoS ONE, 2011-09-08;6(9):e24559.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Group B Streptococcus suppression of phagocyte functions by protein-mediated engagement of human Siglec-5.
Authors: Carlin AF, Chang YC, Areschoug T, Lindahl G, Hurtado-Ziola N, King CC, Varki A, Nizet V
J. Exp. Med., 2009-07-13;206(8):1691-9.
Species: Human
Sample Types: Recombinant Protein
Applications: Western Blot
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