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Human Siglec-5/Siglec-14 Antibody

Catalog #: MAB10721
7 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB10721
MAB10721-SP
Detection of Siglec‑5/14 in Human Monocytes by Flow Cytometry.
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Citations (7)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Siglec-5/Siglec-14 Antibody Summary

Specificity
Detects human Siglec‑5/Siglec‑14 in ELISAs. In sandwich immunoassays, 100% cross-reactivity with recombinant human (rh) Siglec-14 is observed and no cross-reactivity with rhSiglec-3, rhSiglec-7, or rhSiglec-9 is observed.
Source
Monoclonal Mouse IgG1 Clone # 194128
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Siglec‑5/Siglec‑14
Lys18-Thr434
Accession # O15389
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Human Siglec-5 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human Siglec‑5/Siglec‑14 Biotinylated Antibody (Catalog # BAM10722)

Standard: Recombinant Human Siglec-5 Fc Chimera Protein, CF (Catalog # 1072-SL)

Neutralization
Measured by its ability to neutralize Siglec‑5-mediated adhesion of human red blood cells. Kelm, S. et al. (1994) Current Biology 4:965. The Neutralization Dose (ND50) is typically 0.04-0.2 µg/mL in the presence of 5 µg/mL Recombinant Human Siglec‑5 Fc Chimera.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Siglec‑5/14 antibody in Human Monocytes antibody by Flow Cytometry. View Larger

Detection of Siglec‑5/14 in Human Monocytes by Flow Cytometry. Human blood derived monocytes were stained with Mouse Anti-Human Siglec-5/14 Monoclonal Antibody (Catalog # MAB10721, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).

Neutralization Cell Adhesion Mediated by Siglec‑5 and Neutralization by Human Siglec‑5/14 Antibody. View Larger

Cell Adhesion Mediated by Siglec‑5 and Neutralization by Human Siglec‑5/14 Antibody. Recombinant Human Siglec-5 Fc Chimera (Catalog # 1072-SL), immobilized onto a microplate, supports the adhesion of human red blood cells in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human Siglec-5 Fc Chimera (5 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Siglec-5/14 Mono-clonal Antibody (Catalog # MAB10721). The ND50 is typically 0.04-0.2 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Siglec-5/Siglec-14

Siglecs (sialic acid binding Ig-like lectins) are a subgroup of the immunoglobulin superfamily that interact with sialic acids in glycoproteins and glycolipids. Siglec-5 binds to alpha-2, 3- and alpha-2, 6-linked sialic acid equally. It occurs as a disulfide-linked dimer of approximately 140 kDa with the highest expression levels in hematopoietic tissues. Siglec-5 has an inhibitory motif within its cytoplasmic domain. Siglec-14 is an activating receptor that shares 99.5% aa sequence identity with Siglec-5 through the first two extracellular Ig domains and displays a similar glycan binding preference.

Long Name
Sialic Acid Binding Ig-like Lectin 5
Entrez Gene IDs
8778 (Human)
Alternate Names
CD170;CD33L2;OBBP2;OB-BP2;Sialic acid-binding Ig-like lectin 5;SIGLEC5;SIGLEC-5; Siglec-5/Siglec-14

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Citations for Human Siglec-5/Siglec-14 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. The Paired Siglecs in Brain Tumours Therapy: The Immunomodulatory Effect of Dexamethasone and Temozolomide in Human Glioma In Vitro Model
    Authors: P Wielgat, N Wawrusiewi, R Czarnomysy, K Rogowski, K Bielawski, H Car
    International Journal of Molecular Sciences, 2021-02-11;22(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Identification of lipophilic ligands of Siglec5 and 14 that modulate innate immune responses
    Authors: R Suematsu, T Miyamoto, S Saijo, S Yamasaki, Y Tada, H Yoshida, Y Miyake
    J. Biol. Chem., 2019-09-24;0(0):.
    Species: Fungus
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Western Blot
  3. Soluble Siglec-14 glycan-recognition protein is generated by alternative splicing and suppresses myeloid inflammatory responses
    Authors: PJ Huang, PY Low, I Wang, SD Hsu, T Angata
    J. Biol. Chem., 2018-10-30;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  4. Siglec-1 is a novel dendritic cell receptor that mediates HIV-1 trans-infection through recognition of viral membrane gangliosides.
    Authors: Izquierdo-Useros N, Lorizate M, Puertas M, Rodriguez-Plata M, Zangger N, Erikson E, Pino M, Erkizia I, Glass B, Clotet B, Keppler O, Telenti A, Krausslich H, Martinez-Picado J
    PLoS Biol, 2012-12-18;10(12):e1001448.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Sialic acid residues play a pivotal role in alpha(1)-acid glycoprotein (AGP)-induced generation of reactive oxygen species in chemotactic peptide pre-activated neutrophil granulocytes.
    Authors: Gunnarsson P, Fornander L, Pahlsson P, Grenegard M
    Inflamm. Res., 2009-08-11;59(2):89-95.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Group B Streptococcus suppression of phagocyte functions by protein-mediated engagement of human Siglec-5.
    Authors: Carlin AF, Chang YC, Areschoug T, Lindahl G, Hurtado-Ziola N, King CC, Varki A, Nizet V
    J. Exp. Med., 2009-07-13;206(8):1691-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Discovery of Siglec-14, a novel sialic acid receptor undergoing concerted evolution with Siglec-5 in primates.
    Authors: Angata T, Hayakawa T, Yamanaka M, Varki A, Nakamura M
    FASEB J., 2006-10-01;20(12):1964-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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