Human Siglec-5/Siglec-14 Antibody Summary
Lys18-Thr434
Accession # O15389
Applications
Human Siglec-5 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![Detection of Siglec‑5/14 antibody in Human Monocytes antibody by Flow Cytometry. Detection of Siglec‑5/14 antibody in Human Monocytes antibody by Flow Cytometry.](https://aeroglobalimagesuat.blob.core.windows.net/images/datasheets/antibody/Siglec-5_Siglec-14_MAB10721_Flow_Cytometry_8322.jpg)
Detection of Siglec‑5/14 in Human Monocytes by Flow Cytometry. Human blood derived monocytes were stained with Mouse Anti-Human Siglec-5/14 Monoclonal Antibody (Catalog # MAB10721, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).
![Cell Adhesion Mediated by Siglec‑5 and Neutralization by Human Siglec‑5/14 Antibody. Cell Adhesion Mediated by Siglec‑5 and Neutralization by Human Siglec‑5/14 Antibody.](https://aeroglobalimagesuat.blob.core.windows.net/images/datasheets/antibody/Siglec-5_Siglec-14_MAB10721_Block_Neutralize_9004.jpg)
Cell Adhesion Mediated by Siglec‑5 and Neutralization by Human Siglec‑5/14 Antibody. Recombinant Human Siglec-5 Fc Chimera (Catalog # 1072-SL), immobilized onto a microplate, supports the adhesion of human red blood cells in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human Siglec-5 Fc Chimera (5 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Siglec-5/14 Mono-clonal Antibody (Catalog # MAB10721). The ND50 is typically 0.04-0.2 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Siglec-5/Siglec-14
Siglecs (sialic acid binding Ig-like lectins) are a subgroup of the immunoglobulin superfamily that interact with sialic acids in glycoproteins and glycolipids. Siglec-5 binds to alpha-2, 3- and alpha-2, 6-linked sialic acid equally. It occurs as a disulfide-linked dimer of approximately 140 kDa with the highest expression levels in hematopoietic tissues. Siglec-5 has an inhibitory motif within its cytoplasmic domain. Siglec-14 is an activating receptor that shares 99.5% aa sequence identity with Siglec-5 through the first two extracellular Ig domains and displays a similar glycan binding preference.
Product Datasheets
Citations for Human Siglec-5/Siglec-14 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
7
Citations: Showing 1 - 7
Filter your results:
Filter by:
-
The Paired Siglecs in Brain Tumours Therapy: The Immunomodulatory Effect of Dexamethasone and Temozolomide in Human Glioma In Vitro Model
Authors: P Wielgat, N Wawrusiewi, R Czarnomysy, K Rogowski, K Bielawski, H Car
International Journal of Molecular Sciences, 2021-02-11;22(4):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Identification of lipophilic ligands of Siglec5 and 14 that modulate innate immune responses
Authors: R Suematsu, T Miyamoto, S Saijo, S Yamasaki, Y Tada, H Yoshida, Y Miyake
J. Biol. Chem., 2019-09-24;0(0):.
Species: Fungus
Sample Types: Whole Cells
Applications: Flow Cytometry, Western Blot -
Soluble Siglec-14 glycan-recognition protein is generated by alternative splicing and suppresses myeloid inflammatory responses
Authors: PJ Huang, PY Low, I Wang, SD Hsu, T Angata
J. Biol. Chem., 2018-10-30;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
Siglec-1 is a novel dendritic cell receptor that mediates HIV-1 trans-infection through recognition of viral membrane gangliosides.
Authors: Izquierdo-Useros N, Lorizate M, Puertas M, Rodriguez-Plata M, Zangger N, Erikson E, Pino M, Erkizia I, Glass B, Clotet B, Keppler O, Telenti A, Krausslich H, Martinez-Picado J
PLoS Biol, 2012-12-18;10(12):e1001448.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Sialic acid residues play a pivotal role in alpha(1)-acid glycoprotein (AGP)-induced generation of reactive oxygen species in chemotactic peptide pre-activated neutrophil granulocytes.
Authors: Gunnarsson P, Fornander L, Pahlsson P, Grenegard M
Inflamm. Res., 2009-08-11;59(2):89-95.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Group B Streptococcus suppression of phagocyte functions by protein-mediated engagement of human Siglec-5.
Authors: Carlin AF, Chang YC, Areschoug T, Lindahl G, Hurtado-Ziola N, King CC, Varki A, Nizet V
J. Exp. Med., 2009-07-13;206(8):1691-9.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Discovery of Siglec-14, a novel sialic acid receptor undergoing concerted evolution with Siglec-5 in primates.
Authors: Angata T, Hayakawa T, Yamanaka M, Varki A, Nakamura M
FASEB J., 2006-10-01;20(12):1964-73.
Species: Human
Sample Types: Whole Cells
Applications: ICC
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human Siglec-5/Siglec-14 Antibody
There are currently no reviews for this product. Be the first to review Human Siglec-5/Siglec-14 Antibody and earn rewards!
Have you used Human Siglec-5/Siglec-14 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image