Home / SHP-1 / Human SHP-1 Antibody

Human SHP-1 Antibody

Catalog #: MAB1878
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1878
MAB1878-SP
SHP‑1 in HeLa Human Cell Line.
1 Image
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SHP-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human SHP-1.
Source
Monoclonal Rat IgG2A Clone # 255402
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human SHP-1
Ala205-Lys595
Accession # P29350
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
3-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry SHP-1 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

SHP‑1 in HeLa Human Cell Line. SHP-1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rat Anti-Human SHP-1 Monoclonal Antibody (Catalog # MAB1878) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SHP-1

Src-Homology 2 domain Phosphatase-1 (SHP-1), also known as Protein Tyrosine Phosphatase 1C (PTP1C), PTPN6, and Hematopoetic Cell Phosphatase (HCPH), is an enzyme that selectively dephosphorylates tyrosine residues in proteins. Spontaneous point mutations in the SHP-1 gene in mice produce the “motheaten” and "motheaten viable” phenotypes that are severely autoimmune and immunodeficient (1). The enzyme is highly expressed in leukocyte cell types (2). SHP-1 has a regulatory region containing two Src homology 2 (SH2) domains that are critical for its binding to ITIM domains in inhibitory immunoreceptors (3). Deletion of the SH2 domains, as in this product, causes a marked increase in phosphatase activity (4). SHP-1 will dephosphorylate a wide variety of proteins, including the EGF receptor (5). A phosphopeptide containing the EGFR (Y992) sequence (R&D Systems, Catalog # ES006) can be used to measure the activity of SHP-1 by detecting the release of phosphate (R&D Systems, Catalog # DY996).

References
  1. Tsui, H.W. et al. (1993) Nature Genet. 4:124.
  2. Matthews, R.J. et al. (1992) Mol. Cell. Biol. 12:2396.
  3. Burshtyn, D.N. et al. (1997) J. Biol. Chem. 272:13066.
  4. Pei, D. et al. (1994) Biochemistry 33:15483.
  5. Tomic, S. et al. (1995) J. Biol. Chem. 270:21277.
Long Name
Src Homology Region 2 Domain Phosphatase 1
Entrez Gene IDs
5777 (Human); 15170 (Mouse); 116689 (Rat)
Alternate Names
HCP; HCPH; HPTP1C; PTP1C; PTP-1C; PTPN6; SHP1; SHP-1; SHP-1L; SH-PTP1

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human SHP-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. The oncoprotein gankyrin promotes the development of colitis-associated cancer through activation of STAT3
    Authors: T Sakurai, H Higashitsu, H Kashida, T Watanabe, Y Komeda, T Nagai, S Hagiwara, M Kitano, N Nishida, T Abe, H Kiyonari, K Itoh, J Fujita, M Kudo
    Oncotarget, 2017-04-11;8(15):24762-24776.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Regulation and function of immunosuppressive molecule human leukocyte antigen G5 in human bone tissue.
    Authors: Deschaseaux F, Gaillard J, Langonne A, Chauveau C, Naji A, Bouacida A, Rosset P, Heymann D, De Pinieux G, Rouas-Freiss N, Sensebe L
    FASEB J, 2013-04-16;27(8):2977-87.
    Species: Human
    Sample Types: Whole Cells
    Applications: ChIP

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human SHP-1 Antibody

There are currently no reviews for this product. Be the first to review Human SHP-1 Antibody and earn rewards!

Have you used Human SHP-1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review