Human SH2D2A Antibody

Catalog # Availability Size / Price Qty
AF6020
AF6020-SP
Detection of Human SH2D2A by Western Blot.
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Product Details
Citations (3)
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Human SH2D2A Antibody Summary

Species Reactivity
Human
Specificity
Detects human SH2D2A in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human SH2D2A
Ile279-Gln389
Accession # Q9NP31
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human SH2D2A antibody by Western Blot. View Larger

Detection of Human SH2D2A by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line (untreated), human primary differentiated Th2 cells and human primary differentiated Th17 cells treated (+) with 1 µg/mL Hamster Anti-Mouse CD3e Monoclonal Antibody (Catalog # MAB484) and 5 µg/mL Rat Anti-Mouse CD28 Monoclonal Antibody (Catalog # MAB4831) for 120 minutes. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human SH2D2A Polyclonal Antibody (Catalog # AF6020) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SH2D2A at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human SH2D2A antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human SH2D2A by Simple WesternTM. Simple Western lane view shows lysate of human primary differentiated Th2 cells loaded at 0.2 mg/mL. A specific band was detected for SH2D2A at approximately 58 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human SH2D2A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6020) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Simple Western Detection of Human Human SH2D2A Antibody by Simple Western View Larger

Detection of Human Human SH2D2A Antibody by Simple Western TIM-3 Association with Intracellular Kinases in CD8+/MART-1+ T cells.TIM-3 co-immunoprecipitation analysis of unactivated and 15 min. stimulation with anti-CD3/CD28 beads (activated). Equivalent amounts of protein (~2mg) were co-immunoprecipitated with pAb anti-TIM-3 antibody and western blot was performed using capillary electrophoresis. Cleared lysate served as a loading control for individual antibody reactivity. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26492563), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SH2D2A

SH2D2A (SH2 domain-containing protein 2A; also TSAd and VRAP) is a 52 kDa protein best described as a multifunctional intracellular adaptor molecule. It is expressed in activated CD4+ and CD8+ T cells, endothelial cells, and fibroblasts. SH2D2A is found in both cytoplasm and nucleus, and has multiple binding partners. After TCR activation, SH2D2A interacts with LCK, prolonging its activity. It also binds to SMAD2 and SMAD3, and constitutively interacts with PI3K and PLC gamma, thus participating in VEGF:KDR signaling. Human SH2D2A is 389 amino acids (aa) in length and contains one SH2 domain (aa 95‑186) and a Pro-rich region (aa 187‑389). Phosphorylation occurs on Y39, Y216 and Y305, and on S217 and S296.

Entrez Gene IDs
9047 (Human); 27371 (Mouse); 310688 (Rat)
Alternate Names
F2771; RIBP; SCAP; SH2 domain containing 2A; SH2 domain protein 2A; SH2 domain-containing adapter protein; SH2D2A; T cell specific adapter protein TSAd; T cell-specific adapter protein; T lymphocyte specific adaptor protein; TSAd; TSADSH2 domain-containing protein 2A; VEGF receptor-associated protein; VRAP

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Citations for Human SH2D2A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. TIM-3 Suppresses Anti-CD3/CD28-Induced TCR Activation and IL-2 Expression through the NFAT Signaling Pathway.
    Authors: Tomkowicz B, Walsh E et al.
    PLoS One
    Species: Human
    Sample Types: blood
    Applications: Simple Western
  2. VEGFR2 induces c-Src signaling and vascular permeability in vivo via the adaptor protein TSAd.
    Authors: Sun Z, Li X, Massena S, Kutschera S, Padhan N, Gualandi L, Sundvold-Gjerstad V, Gustafsson K, Choy WW, Zang G, Quach M, Jansson L, Phillipson M, Abid MR, Spurkland A, Claesson-Welsh L
    J. Exp. Med., 2012-06-11;209(7):1363-77.
    Species: Human, Mouse
    Sample Types: Cell Lysates, Tissue Homogenates
    Applications: Immunoprecipitation, Western Blot
  3. Axl is essential for VEGF-A-dependent activation of PI3K/Akt.
    Authors: Ruan GX, Kazlauskas A
    EMBO J., 2012-02-10;31(7):1692-703.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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