Human Secretagogin Antibody

Catalog # Availability Size / Price Qty
MAB4878
MAB4878-SP
Detection of Human and Mouse Secretagogin by Western Blot.
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Human Secretagogin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Secretagogin in ELISAs and Western Blots. Detects mouse Secretagogin in Western Blots
Source
Monoclonal Mouse IgG1 Clone # 778518
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Secretagogin
Asn164-Pro276
Accession # O76038
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse Secretagogin antibody by Western Blot. View Larger

Detection of Human and Mouse Secretagogin by Western Blot. Western blot shows lysates of beta TC-6 mouse beta cell insulinoma cell line and human pancreas tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Secretagogin Monoclonal Antibody (Catalog # MAB4878) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Secretagogin at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Secretagogin antibody in Human Pancreas by Immunohistochemistry (IHC-P). View Larger

Secretagogin in Human Pancreas. Secretagogin was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human Secretagogin Monoclonal Antibody (Catalog # MAB4878) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Human and Mouse Secretagogin antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human and Mouse Secretagogin by Simple WesternTM. Simple Western lane view shows lysates of beta TC‑6 mouse beta cell insulinoma cell line and human pancreas tissue, loaded at 0.5 mg/mL. A specific band was detected for Secretagogin at approximately 37 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Secretagogin Monoclonal Antibody (Catalog # MAB4878). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Secretagogin

Secretagogin (SCGN) is a 32 kDa member of the EF-hand family of Ca-binding proteins. It is expressed in select neurons, as well as neuroendocrine cells and pancreatic islets. Human Secretagogin is 276 amino acids (aa) in length and contains six 35 aa EF-hand domains (aa 12‑276). There is one alternative splice variant that shows a 22 aa substitution for aa 28‑276, and which demonstrates no Ca-binding ability. Secretagogin is known to circulate, presumably released during cell apoptosis. Over aa 164‑276, human Secretagogin is more than 97% aa identical to mouse, porcine and canine Secretagogin.

Entrez Gene IDs
10590 (Human); 214189 (Mouse); 306942 (Rat)
Alternate Names
CALBL; DJ501N12.8; SCGN; SECRET; Secretagogin; secretagogin, EF-hand calcium binding protein; SECRETsecretagogin; SEGN; Setagin

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