Human S1P3/EDG-3 Antibody

Catalog #: MAB7524 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7524
MAB7524-SP

Detection of S1P3/EDG-3 in S1P3/EDG-3 and eGFP Co-Transfected Cells by Flow Cytometry.

1 Image

All S1P3/EDG-3 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human S1P3/EDG-3 Antibody Summary

Specificity

Detects human S1P₃/EDG‑3 in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 776808

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human S1P3/EDG-3 transfected NS0 cells

Accession # Q99500

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of S1P₃/EDG-3 in S1P₃/EDG-3 and eGFP Co-Transfected Cells by Flow Cytometry. Human S1P₃/EDG-3 and eGFP co-transfectants were stained with either (A) Mouse IgG2A Isotype Control (Catalog # MAB003) or (B) Mouse Anti-Human S1P₃/EDG-3 Monoclonal Antibody (Catalog # MAB7524) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: S1P3/EDG-3

Sphingosine 1-Phosphate receptor-3 (S1P3), also known as EDG-3, is a 378 amino acid (aa) seven-transmembrane receptor for the lysolipid phosphoric acid mediator, sphingosine 1-phosphate (S1P). S1P3 interactions with S1P induce a range of responses including vasoconstriction, brachycardia, vascular endothelial and smooth muscle cell proliferation and migration, heart and liver fibrosis, and coagulation-induced inflammation. Extracellular portions of human S1P3 show 74% aa identity with mouse and rat S1P3.

Long Name

Endothelial Differentiation Gene 3

Entrez Gene IDs

1903 (Human); 13610 (Mouse); 306792 (Rat)

Alternate Names

EDG3; EDG-3; EDG3FLJ37523; Endothelial differentiation G-protein coupled receptor 3; endothelial differentiation, sphingolipid G-protein-coupled receptor, 3; FLJ93220; G protein-coupled receptor, endothelial differentiation gene-3; LPB3; MGC71696; S1P receptor 3; S1P receptor EDG3; S1P receptor Edg-3; S1P3; S1PR3; sphingosine 1-phosphate receptor 3; Sphingosine 1-phosphate receptor Edg-3; sphingosine-1-phosphate receptor 3