Human S1P3/EDG-3 Antibody Summary
Accession # Q99500
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![S1P<SUB>3</SUB>/EDG-3 antibody in Human Spleen by Immunohistochemistry (IHC-P). S1P<SUB>3</SUB>/EDG-3 antibody in Human Spleen by Immunohistochemistry (IHC-P).](https://aeroglobalimagesuat.blob.core.windows.net/images/datasheets/antibody/S1P3_MAB75241_Immunohistochemistry_12337.jpg)
S1P3/EDG‑3 in Human Spleen. S1P3/EDG-3 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human S1P3/EDG-3 Monoclonal Antibody (Catalog # MAB75241) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the marginal zone of germinal centers. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: S1P3/EDG-3
EDG-3, also known as sphingosine 1-phosphate receptor-3 (S1P3), is a 378 amino acid (aa) seven-transmembrane receptor for the lysolipid phosphoric acid mediator, sphingosine 1-phosphate (S1P). EDG-3 interactions with S1P induce a range of responses including vasoconstriction, brachycardia, vascular endothelial and smooth muscle cell proliferation and migration, heart and liver fibrosis, and coagulation-induced inflammation. Extracellular portions of human EDG-3 show 74% aa identity with mouse and rat EDG-3.
Product Datasheets
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