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Human S1P4/EDG-6 Antibody

Catalog #: MAB10321 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB10321-100
MAB10321-SP
Detection of S1P4/EDG-6 in HEK293 Human Cell Line Transfected with Human S1P4/EDG-6 and eGFP by Flow Cytometry.
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Human S1P4/EDG-6 Antibody Summary

Species Reactivity
Human
Specificity
Detects human S1P4/EDG‑6 transfectants but not the parental cell line in Flow Cytometry.
Source
Monoclonal Mouse IgG2B Clone # 1012512
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human S1P4/EDG‑6
Met1-Ile384
Accession # O95977
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of S1P4/EDG-6 antibody in HEK293 Human Cell Line Transfected with Human S1P4/EDG-6 and eGFP antibody by Flow Cytometry. View Larger

Detection of S1P4/EDG-6 in HEK293 Human Cell Line Transfected with Human S1P4/EDG-6 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human S1P4/EDG-6 or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human S1P4/EDG-6 Monoclonal Antibody (Catalog # MAB10321) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: S1P4/EDG-6

EDG-6 belongs to the G protein-coupled receptor 1 family. It is specifically expressed in fetal and adult lymphoid and hematopoietic tissue as well as in lung. EDG-6 is a receptor for the lysosphingolipid sphingosine 1- phosphate (S1P), a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. EDG-6 may be involved in cell migration processes that are specific for lymphocytes.

Long Name
Endothelial Differentiation Gene 5
Entrez Gene IDs
8698 (Human); 13611 (Mouse); 314649 (Rat)
Alternate Names
EDG6; EDG6S1P receptor Edg-6; Endothelial differentiation G-protein coupled receptor 6; endothelial differentiation, G-protein-coupled receptor 6; endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor6,endothelial differentiation, G protein coupled receptor 6; S1P receptor 4; S1P4; S1P4LPC1; S1PR4; SLP4; sphingosine 1-phosphate receptor 4; Sphingosine 1-phosphate receptor Edg-6; sphingosine-1-phosphate receptor 4

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