Human ROR gamma /RORC/NR1F3 Antibody

Catalog #: MAB61092 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB61092
MAB61092-SP

Detection of Human ROR gamma /RORC/NR1F3 by Western Blot.

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Human ROR gamma /RORC/NR1F3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ROR gamma /RORC/NR1F3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) ROR1, rhROR2, rhROR gamma t, or rhROR alpha is observed.

Source

Monoclonal Mouse IgG_2B Clone # 732111

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human ROR gamma /RORC/NR1F3
Met1-Gln121
Accession # P51449

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human ROR gamma /RORC/NR1F3 by Western Blot. Western blot shows lysates of human Th1 cells. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human ROR gamma/RORC/NR1F3 Monoclonal Antibody (Catalog # MAB61092) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ROR gamma/RORC/NR1F3 at approximately 67 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ROR gamma/RORC/NR1F3

ROR gamma (Nuclear receptor ROR-gamma), also called RORC and NR1F3, is a member of the NR1 nuclear hormone receptor family. ROR gamma is a DNA binding transcription factor. ROR gamma is 518 amino acids (aa) in length. Deletion in mice implicates ROR gamma as being essential for lymphoid organogenesis and controlling apoptosis during thymopoiesis. Two splice forms differing in the first 24 aa have been found for this gene; isoform 2 deletes aa 1‑21 resulting in an alteration of aa 22‑24. Over aa 1‑121 human ROR gamma shares 97% identity with mouse ROR gamma.

Long Name

Retinoic Acid-Related Orphan Receptor gamma

Entrez Gene IDs

6097 (Human)

Alternate Names

MGC129539; NR1F3; Nuclear receptor ROR gamma; Nuclear receptor ROR-gamma; Nuclear receptor RZR gamma; Nuclear receptor RZR-gamma; Nuclear receptor subfamily 1 group F member 3; RAR-Related Orphan Nuclear Receptor Variant 2; RAR-related orphan receptor C; RAR-related orphan receptor gamma; retinoic acid-binding receptor gamma; retinoid-related orphan receptor gamma; Retinoid-related orphan receptor-gamma; ROR gamma; RORC; RORG; RORGMGC129539; RZRG; RZRGRZR-GAMMA; TOR