Human RNF4 Antibody Summary
Met1-Ile190
Accession # P78317
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human RNF4 by Western Blot. Western blot shows lysates of GM00719D human ataxia telangiectasia cell line either mock-treated (-) or exposed (+) to 10 Gy ionizing radiation (IR) and harvested after 1 hour, BaF3 mouse pro-B cell line, and LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human RNF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7964) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for RNF4 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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RNF4 in HeLa Human Cell Line. RNF4 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human RNF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7964) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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RNF4 in Human Testis. RNF4 was detected in immersion fixed paraffin-embedded sections of human testis using Goat Anti-Human RNF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7964) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RNF4
RNF4 (small nuclear ring finger protein, SNURF) is a RING-finger ubiquitin E3 ligase that ubiquitinates and mediates the proteasomal destruction of targets such as PML, PEA3, CENP1, and PARP1. In addition to the RING domain, RNF4 contains four SUMO-interacting motifs (SIMs) that function to recruit this ligase to poly-sumoylated substrates. RNF4 will autoubiquitinate in vitro, and will also ubiquitinate poly-SUMO chains.
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Geoffroy, M-C et al. (2010) Mol. Bio. Cell 21: 4227
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Tathum, M.H. et al. (2008) Nat. Cell Bio. 10: 538
Product Datasheets
Citations for Human RNF4 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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WRN inhibition leads to its chromatin-associated degradation via the PIAS4-RNF4-p97/VCP axis
Authors: Rodríguez Pérez, F;Natwick, D;Schiff, L;McSwiggen, D;Heckert, A;Huey, M;Morrison, H;Loo, M;Miranda, RG;Filbin, J;Ortega, J;Van Buren, K;Murnock, D;Tao, A;Butler, R;Cheng, K;Tarvestad, W;Zhang, Z;Gonzalez, E;Miller, RM;Kelly, M;Tang, Y;Ho, J;Anderson, D;Bashore, C;Basham, S;
Nature communications
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
RAD54L2-mediated DNA damage avoidance pathway specifically preserves genome integrity in response to topoisomerase 2 poisons
Authors: Zhang, H;Xiong, Y;Sun, Y;Park, JM;Su, D;Feng, X;Keast, S;Tang, M;Huang, M;Wang, C;Srivastava, M;Yang, C;Zhu, D;Chen, Z;Li, S;Yin, L;Pommier, Y;Chen, J;
Science advances
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Atypical genotoxicity of carcinogenic nickel(II): linkage to dNTP biosynthesis, DNA-incorporated rNMPs and impaired repair of TOP1-DNA crosslinks
Authors: Krawic, C;Luczak, MW;Valiente, S;Zhitkovich, A;
The Journal of biological chemistry
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
RNF4 and USP7 cooperate in ubiquitin-regulated steps of DNA replication
Authors: Chang, YC;Lin, K;Baxley, RM;Durrett, W;Wang, L;Stojkova, O;Billmann, M;Ward, H;Myers, CL;Bielinsky, AK;
Open biology
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
SPRTN patient variants cause global-genome DNA-protein crosslink repair defects
Authors: P Weickert, HY Li, MJ Götz, S Dürauer, D Yaneva, S Zhao, J Cordes, AC Acampora, I Forne, A Imhof, J Stingele
Nature Communications, 2023-01-21;14(1):352.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blots -
Estradiol-mediated inhibition of Sp1 decreases miR-3194-5p expression to enhance CD44 expression during lung cancer progression
Authors: MJ Young, YC Chen, SA Wang, HP Chang, WB Yang, CC Lee, CY Liu, YL Tseng, YC Wang, HS Sun, WC Chang, JJ Hung
Journal of Biomedical Science, 2022-01-17;29(1):3.
Species: Xenograft
Sample Types: Cell Lysates
Applications: Western Blot -
Inhibiting ubiquitination causes an accumulation of SUMOylated newly synthesized nuclear proteins at PML bodies
Authors: Z Sha, T Blyszcz, R González-P, ACO Vertegaal, AL Goldberg
J. Biol. Chem., 2019-07-08;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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