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Human Reg1A Antibody

Catalog #: MAB4937
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB4937
MAB4937-SP
Detection of Reg1A in AGS Human Cell Line by Flow Cytometry.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Reg1A Antibody Summary

Species Reactivity
Human
Specificity
Detects human Reg1A in Western blots. Shows 5% cross-reactivity with recombinant human (rh) Reg1B and no cross-reactivity with rhReg3A, recombinant mouse Reg1, or recombinant rat Reg1.
Source
Monoclonal Rat IgG2B Clone # 431202
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Reg1A
Gln23-Asn166
Accession # P05451
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Reg1A
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of Reg1A antibody in AGS Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Reg1A in AGS Human Cell Line by Flow Cytometry. AGS human gastric adenocarcinoma cell line was stained with Rat Anti-Human Reg1A Monoclonal Antibody (Catalog # MAB4937, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Reg1A

Reg1A, also known as PTP, PSP, and lithostathine, is a member of the Reg family of secreted proteins with a C-type lectin domain. Due to variable glycosylation, pancreatic Reg1A exists as multiple species of 16‑18 kDa. Reg1A promotes the maintenance and growth of pancreatic islet beta -cells and intestinal villi. It is up‑regulated in pancreatitis and some carcinomas. Reg1A is an antigenic target in autoimmune diabetes. Human Reg1A shares 65%‑68% aa sequence identity with mouse and rat Reg1A.

Long Name
Regenerating Islet-derived 1A
Entrez Gene IDs
5967 (Human)
Alternate Names
ICRF; Islet cells regeneration factor; Islet of Langerhans regenerating protein; lithostathine-1-alpha; P19; pancreatic stone protein, secretory; Pancreatic thread protein; protein-X; PSP; PSPS; PSPS1; PTP; REG; Reg1A; REG-1-alpha; Regenerating islet-derived protein 1-alpha; Regenerating protein I alpha

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Citations for Human Reg1A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Antimicrobial overproduction sustains intestinal inflammation by inhibiting Enterococcus colonization
    Authors: KK Jang, T Heaney, M London, Y Ding, F Yeung, D Ercelen, YH Chen, J Axelrad, S Gurunathan, A Marijke Ke, ME Griffin, HC Hang, K Cadwell
    bioRxiv : the preprint server for biology, 2023-02-01;0(0):.
    Species: Human, Mouse
    Sample Types: Stool Extracts, Tissue Homogenates
    Applications: Western Blot
  2. Ulcerative colitis mucosal transcriptomes reveal mitochondriopathy and personalized mechanisms underlying disease severity and treatment response
    Authors: Y Haberman, R Karns, PJ Dexheimer, M Schirmer, J Somekh, I Jurickova, T Braun, E Novak, L Bauman, MH Collins, A Mo, MJ Rosen, E Bonkowski, N Gotman, A Marquis, M Nistel, PA Rufo, SS Baker, CG Sauer, J Markowitz, MD Pfefferkor, JR Rosh, BM Boyle, DR Mack, RN Baldassano, S Shah, NS Leleiko, MB Heyman, AM Grifiths, AS Patel, JD Noe, BJ Aronow, S Kugathasan, TD Walters, G Gibson, SD Thomas, K Mollen, S Shen-Orr, C Huttenhowe, RJ Xavier, JS Hyams, LA Denson
    Nat Commun, 2019-01-03;10(1):38.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC

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