Human Reduced Folate Carrier/SLC19A1 Antibody

Catalog #: MAB8450 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8450
MAB8450-SP

Detection of Reduced Folate Carrier/SLC19A1 in HEK293 Human Cell Line Transfected with Human Reduced Folate Carrier/SLC19A1 and eGFP by Flow Cytometry.

1 Image

All Reduced Folate Carrier/SLC19A1 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Reduced Folate Carrier/SLC19A1 Antibody Summary

Species Reactivity

Human

Specificity

Stains human Reduced Folate Carrier/SLC19A1 transfectants but not irrelevant transfectants in flow cytometry.

Source

Monoclonal Mouse IgG_2A Clone # 890513

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human Reduced Folate Carrier/SLC19A1
Met1-Gln591
Accession # P41440

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

Detection of Reduced Folate Carrier/SLC19A1 antibody in HEK293 Human Cell Line Transfected with Human Reduced Folate Carrier/SLC19A1 and eGFP antibody by Flow Cytometry.

View Larger

Detection of Reduced Folate Carrier/SLC19A1 in HEK293 Human Cell Line Transfected with Human Reduced Folate Carrier/SLC19A1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line either (A) transfected with human Reduced Folate Carrier/SLC19A1 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Reduced Folate Carrier/SLC19A1 Monoclonal Antibody (Catalog # MAB8450) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Reduced Folate Carrier/SLC19A1

SLC19A1, also known as, Reduced Folate Carriers, is a transmembrane transporter that is involved in the regulation of intracellular concentrations of folate. There is an association between idiopathic recurrent spontaneous abortion (RSA) and 3 SLC19A1 polymorphisms that may be useful biomarkers for RSA risk. Three transcript variants encoding different isoforms have been found for this gene.

Long Name

Solute Carrier Family 12 (Folate Transporter) Member 3

Entrez Gene IDs

6573 (Human); 20509 (Mouse); 29723 (Rat)

Alternate Names

CHMD; FLOT1; folate transporter 1; FOLT; FOLThuman reduced folate carrier (RFC)10Intestinal folate carrier 1; IFC1; IFC-1; Placental folate transporter; Reduced folate carrier protein; REFC; RFC; RFC1; SLC19A1; solute carrier family 19 (folate transporter), member 1; Solute carrier family 19 member 1