Human/Rat SOX10 Antibody

SOX10 in BG01V Human Embryonic Stem Cells. SOX10 was detected in immersion fixed BG01V human embryonic stem cells differentiated to neural crest stem cells using Mouse Anti-Human/Rat SOX10 Monoclonal Antibody (Catalog # MAB2864) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

SOX10 in Human Melanoma Tissue. SOX10 was detected in immersion fixed paraffin-embedded sections of human melanoma tissue using Mouse Anti-Human/Rat SOX10 Monoclonal Antibody (Catalog # MAB2864) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Rat SOX10 by Immunocytochemistry/Immunofluorescence Identification of neural crest-derived DPCs. ((a)–(c)) Immunofluorescent cytochemical staining revealed expression of NCSCs-specific markers in DPCs. Primary DPCs were double positive for P75 and Sox10 (a), P75 and Nestin (b), and were positive for Sox9 (c). (d) RT-PCR analysis confirmed the expression of dermal papillae markers ALP and Sox2, and the NCSCs markers Nestin, P75, Sox9, Twist1, and AP2 alpha in DPCs, as well as BMSCs. Scale bar = 20 μm ((a)–(c)). DPCs-P#: DPCs at passage #; BMSCs-P#: BMSCs at passage #. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25045659), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse SOX10 by Immunocytochemistry/Immunofluorescence JCV T-antigen is expressed in neural crest cells.Fluorescent images of bone marrow cells isolated from JCV T-antigen transgenic mice cultured in neural conditions show that these cells are positive for p75 (A,B), nestin (D,E) and SOX-10 (G,H), which are all markers of the neural crest. JCV T-antigen (J,K) was detected in the nucleus of p75 positive cells. Bone marrow cells cultured in mesenchymal media were negative for p75, nestin and SOX-10, and did not express JCV T-antigen (C, F, I, L). Cell nuclei were visualized with DAPI. (Scale bar: 100 µm). (M) Diagram illustrating expression of JCV T-antigen in bone marrow cells. T-antigen expression was associated with neural crest lineage cells of the bone marrow cultured in serum-free neural stem cell media supplemented with bFGF and EFG (left panel), while mesenchymal cells maintained in alpha -MEM in the presence of 20% serum were negative for T-antigen (right panel). (N) FACS analysis of neural crest cells and MSCs demonstrates positivity for JCV T-antigen in 99% of neural crest cells and lack of JCV T-antigen expression in MSCs. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23805194), licensed under a CC-BY license. Not internally tested by R&D Systems.