Are there special considerations for Flow Cytometry using Human CCR8 Antibody, Catalog # MAB1429 and FAB1429?

Name: Human/Rat CCR8 PerCP-conjugated Antibody
Brand: R&D Systems
SKU: FAB1429C
Price: 169 USD

The staining with this antibody is temperature dependent; it will stain at 4 C and room temperature, however, staining is stronger at 37 C.

Human/Rat CCR8 PerCP-conjugated Antibody

Catalog #: FAB1429C Datasheet / COA / SDS

Clone 191704 was used by HLDA to establish CD designation

Catalog #	Availability	Size / Price	Qty
FAB1429C-025
FAB1429C-100

Detection of CCR8 in Human Blood Monocytes by Flow Cytometry.

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Human/Rat CCR8 PerCP-conjugated Antibody Summary

Species Reactivity

Human, Rat

Specificity

Detects human CCR8. Neutralizes the bioactivity of human CCR8 and specifically stains human CCR8-transfected cell lines but not the parent cell lines.

Source

Monoclonal Rat IgG_2B Clone # 191704

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

BaF3 mouse pro-B cell line transfected with human CCR8
Met1-Leu355
Accession # P51685

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CCR8 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Rat Anti-Human/Rat CCR8 PerCP-conjugated Monoclonal Antibody (Catalog # FAB1429C, filled histogram) or isotype control antibody (Catalog # IC013C, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of CCR8 in Rat Peripheral Blood Cells by Flow Cytometry. Rat peripheral blood cells were stained with Rat Anti-Human/Rat CCR8 PerCP-conjugated Monoclonal Antibody (Catalog # FAB1429C, filled histogram) or isotype control antibody (Catalog # IC013C, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR8

CCR8 (C-C chemokine receptor 8; previously called CKR-L1 or TER1 and designated CD198) is a 41-43 kDa member of the GPCR 1 family of transmembrane proteins. CCR8 is expressed on vascular smooth muscle cells, monocytes, eosinophils, peritoneal macrophages, thymocytes, CD8⁺ T cells, Langerhans cells and neurons. CCL1/TCA3 and vMIP-1 are known agonists for CCR8. Human CCR8 is a 7-transmembrane protein that is 355 amino acids (aa) in length. It contains a 35 aa N-terminal extracellular domain plus a 51 aa C-terminal cytoplasmic tail. Over aa sequences 1-33 and 92-105 collectively, human and mouse CCR8 share 64% aa sequence identity.

Entrez Gene IDs

1237 (Human); 12776 (Mouse)

Alternate Names

CC chemokine receptor 8; C-C CKR-8; CC-chemokine receptor chemr1; CC-CKR-8; CCR8; CCR-8; CD198; CDw198 antigen; CDw198; chemokine (C-C motif) receptor 8; chemokine (C-C) receptor 8; chemokine (C-C) receptor-like 2; Chemokine receptor-like 1; ChemR1; CKRL1; CKR-L1; CKRL1MGC129966; CMKBR8MGC129973; CMKBRL2C-C chemokine receptor type 8; CY6; GPRCL1; GPRCY6; GPR-CY6; TER-1; TER1CC chemokine receptor CHEMR1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

FAQs

Are there special considerations for Flow Cytometry using Human CCR8 Antibody, Catalog # MAB1429 and FAB1429?
- The staining with this antibody is temperature dependent; it will stain at 4 C and room temperature, however, staining is stronger at 37 C.

View all Antibody FAQs