Human R-Spondin 3 Antibody

Catalog #: MAB3500 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3500-100
MAB3500-SP

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All R-Spondin 3 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human R-Spondin 3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human R-Spondin 3 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 1028220

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese Hamster Ovary cell line CHO-derived human R-Spondin 3
Gln22-His272
Accession # Q9BXY4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human R‑Spondin 3 Monoclonal Antibody (Catalog # MAB35001).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human R-Spondin 3 DuoSet ELISA Kit (Catalog # DY3500) for convenient development of a sandwich ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human R‑Spondin 3 ELISA Standard Curve. Recombinant Human R‑Spondin 3 protein was serially diluted 2-fold and captured by Mouse Anti-Human R‑Spondin 3 Monoclonal Antibody (MAB35001) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human R‑Spondin 3 Monoclonal Antibody (Catalog # MAB3500) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: R-Spondin 3

R-Spondin 3 (RSPO3, roof plate-specific spondin 3), also called cysteine-rich and single thrombospondin domain containing-1 (Cristin 1), is an ~31 kDa secreted protein that shares ~40% amino acid (aa) identity with the other three R-Spondin family members (1, 2). All are positive modulators of Wnt/ beta -catenin signaling, but each has a distinct expression pattern (1-4). Like other R-spondins,R-Spondin 3 contains two adjacent cysteine-rich furin-like domains (aa 35-135) with one potential N-glycosylation site (aa 36), followed by a thrombospondin (TSP-1) motif (aa 147-207) and a region rich in basic residues (aa 211-269). Only the furin-like domains are needed for beta -catenin stabilization (2). Within aa 21-209, human R-Spondin 3 shares 93%, 92%, 97%, 96% and 92% aa identity with mouse, rat, equine, bovine and canine R-Spondin 3, respectively. Potential isoforms of 279 and 297 aa diverge at aa 210 and 276, respectively (5). Mouse R-Spondin 3 is critical for development of the placental labyrinthine layer, probably by promoting VEGF expression and thus vascular development (6, 7). It is also essential for expression of the placenta-specific transcription factor, Gcm1. In the mouse embryo, R-Spondin 3 is often expressed by or located near endothelial cells (6). It is found in the roof plate, tail, somites, otic vesicles, cephalic mesoderm, truncus arteriosus, atrioventricular canal of the developing heart, and strongly but transiently in developing limbs (4, 7). R-Spondins regulate Wnt/ beta -catenin by competing with the Wnt antagonist DKK-1 for binding to the Wnt co-receptors LRP-6 and Kremen, reducing their DKK-1-mediated internalization (8, 9). Reports differ on whether R-Spondins bind LRP-6 directly (8-10). R-Spondin 3 has also been identified as an oncogene (11).

References

Chen, J-Z. et al. (2002) Mol. Biol. Rep. 29:287.
Kim, K.-A. et al. (2008) Mol. Biol. Cell 19:2588.
Hendrickx, M. and L. Leyns (2008) Develop. Growth Differ. 50:229.
Nam, J.-S. et al. (2007) Gene Expr. Patterns 7:306.
Entrez Accession # EAW48114 and EAW48116.
Kazanskaya, O. et al. (2008) Development 135:3655.
Aoki, M. et al. (2007) Dev. Biol. 301:218.
Binnerts, M.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14700.
Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247.
Wei, Q. et al. (2007) J. Biol. Chem. 282:15903.
Theodorou, V. et al. (2007) Nat. Genet. 6:759.

Long Name

Roof Plate-specific Spondin 3

Entrez Gene IDs

84870 (Human); 72780 (Mouse); 102132009 (Cynomolgus Monkey)

Alternate Names

Cristin 1; CRISTIN1; FLJ14440; hPWTSR; hRspo3; Protein with TSP type-1 repeat; PWTSR; Roof plate-specific spondin-3; RSPO3; R-spondin 3 homolog (Xenopus laevis); RSpondin 3; R-Spondin 3; R-spondin-3; Thrombospondin type-1 domain-containing protein 2; thrombospondin, type I, domain containing 2; THSD2