Human PXR/NR1I2 Antibody

Catalog #: PP-H0502-00 Datasheet / COA / SDS

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PP-H0502-00

All PXR/NR1I2 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PXR/NR1I2 Antibody Summary

Species Reactivity

Human

Specificity

This antibody specifically recognizes human PXR2 but not human PXR1. The antibody epitope lies in amino acids 1-40. Not yet tested in other species.

Source

Monoclonal Mouse IgG_2A Clone # H0502

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Recombinant human PXR/NR1I2
aa 1-78

Formulation

A liquid formulation in physiologic saline with 0.1% NaN₃.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

This antibody can be used at 1-10 μg/mL under reducingconditions and at 3-10 μg/mL under non-reducing conditions with theappropriate secondary reagents to detect human PXR2.

Direct ELISA

This antibody can be used at 0.5 μg/mL with the appropriatesecondary reagents to detect PXR2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

This antibody is stable for greater than six months when stored at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Background: PXR/NR1I2

Pregnane X Receptor 2 (PXR2; also known as PAR2, SXR and NR1I2) is a 46 kDa member of the NR1 subfamily of the Nuclear Hormone Receptor family. It is one of at least seven splice forms from a single gene. Two basic forms exist, a 434 amino acid (aa) PXR1 form and a 473 aa PXR2 form. The PXR2 isoform has a 39 aa extension at the N-terminus and results from the use of an alternate exon 1. PXR forms heterodimers with Retinoid X Receptor (RXR) and is activated by pregnanolone, progesterone, xenobiotics, and endobiotics.

Long Name

Pregnane X Receptor

Entrez Gene IDs

8856 (Human); 18171 (Mouse)

Alternate Names

BXR; NR1I2; nuclear receptor subfamily 1 group I member 2; nuclear receptor subfamily 1, group I, member 2; ONR1; Orphan nuclear receptor PAR1; Orphan nuclear receptor PXR; PAR; PAR1; PAR2; Pregnane X receptor; PRR; PXR; PXRPARq; SAR; Steroid and xenobiotic receptor; SXR; SXRBXR