Human PTGER1 Antibody

Name: Human PTGER1 Antibody
Brand: R&D Systems
SKU: MAB100081
Rating: 5 (1 reviews)

Catalog #: MAB100081 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB100081-100
MAB100081-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PTGER1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human PTGER1 in direct ELISAs.

Source

Monoclonal Mouse IgG2b Clone # 1014918

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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PTGER1 in Human Breast Cancer Tissue. PTGER1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human PTGER1 Monoclonal Antibody (Catalog # MAB100081) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: PTGER1

PTGER1 is a one of four EP₁ receptors for PGE2 which is mediated by G(q) proteins. PTGER1 may play a role as an important modulator of renal functions. It has also been detected in mast cells, pulmonary veins, keratinocytes, myometrium and colon smooth muscle. EP₁ expression has also frequently been seen in human breast cancers. Nuclear expression of EP₁ correlates with good prognosis markers like node negative status and PR expression. Animal model studies have implicated EP₁in various physiological and pathological responses however expression in humans appears to be more limited. EP₁receptor antagonists have been studied clinically primarily to treat hyperalgesia.

Long Name

Prostaglandin E2 Receptor 1, Subtype EP1

Entrez Gene IDs

5731 (Human); 19216 (Mouse); 25637 (Rat)

Alternate Names

EP1; PGE receptor EP1 subtype; PGE receptor, EP1 subtype; PGE2 receptor EP1 subtype; prostaglandin E receptor 1 (subtype EP1), 42kD; prostaglandin E receptor 1 (subtype EP1), 42kDa; prostaglandin E receptor 1, subtype EP1; prostaglandin E2 receptor EP1 subtype; Prostanoid EP1 receptor; PTGER1