Human PSMA/FOLH1/NAALADase I Antibody

Catalog #: MAB42341
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB42341-100
MAB42341-SP

Human PSMA/FOLH1/NAALADase I ELISA Standard Curve.

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Human PSMA/FOLH1/NAALADase I Antibody Summary

Species Reactivity

Human

Specificity

Detects human PSMA/FOLH1/NAALADase I in sandwich immunoassays.

Source

Monoclonal Mouse IgG₁ Clone # 460424

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human PSMA/FOLH1/NAALADase I
Lys44-Ala750
Accession # Q04609

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Sheep Anti-Human PSMA/FOLH1/NAALADase I Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4234).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human PSMA/FOLH1 DuoSet ELISA Kit (Catalog # DY4234-05) for convenient development of a sandwich ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human PSMA/FOLH1/NAALADase I ELISA Standard Curve. Recombinant Human PSMA/FOLH1/NAALADase I protein was serially diluted 2-fold and captured by Mouse Anti-Human PSMA/FOLH1/NAALADase I Monoclonal Antibody (Catalog # MAB42341) coated on a Clear Polystyrene Microplate (DY990). Sheep Anti-Human PSMA/FOLH1/NAALADase I Antigen Affinity-purified Polyclonal Antibody (AF4234) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PSMA/FOLH1/NAALADase I

Human prostate-specific membrane antigen (PSMA), a tumor marker in prostate cancer encoded by the FOLH1 gene, is a type II transmembrane zinc metallopeptidase that is most highly expressed in the nervous system, prostate, kidney, and small intestine (1, 2). The enzyme is also known as glutamate carboxypeptidase II (GCPII), folate hydrolase 1, folypoly-gamma-glutamate carboxypeptidase (FGCP), and N-acetylated-alpha-linked acidic dipeptidase I (NAALADase I). In the brain, PSMA hydrolyzes the neurotransmitter N-acetyl-Asp-Glu to produce glutamate, another neurotransmitter. Inhibition of brain PSMA activity is considered to be a promising approach for the treatment of neurological disorders associated with glutamate excitotoxicity, such as stroke, chronic pain, and amyotrophic lateral sclerosis (3). Intestinal PSMA hydrolyzes folylpoly-gamma -glutamates, facilitating the uptake of folate (4).

References

Silver, D.A. et al. (1997) Clin. Cancer Res. 3:81.
Carter, R.E. et al. (1996) Pro. Natl. Acad. Sci. USA. 93:749.
Jackson, P.F. and Slusher, B.S. (2001) Curr. Med. Chem. 8:949.
Heston, W.D. (1997) Urology 49:104.

Long Name

Prostate-specific Membrane Antigen

Entrez Gene IDs

2346 (Human); 53320 (Mouse); 85309 (Rat); 101866140 (Cynomolgus Monkey)

Alternate Names

Cell growth-inhibiting gene 27 protein; cell growth-inhibiting protein 27; EC 3.4.17.21; FGCP; folate hydrolase (prostate-specific membrane antigen) 1; Folate hydrolase 1; FOLH1; FOLHNAALADase I; Folylpoly-gamma-glutamate carboxypeptidase; GCP2; GCPII; GCPIImGCP; glutamate carboxylase II; glutamate carboxypeptidase 2; Glutamate carboxypeptidase II; Membrane glutamate carboxypeptidase; mopsm; NAALAD1; NAALAD1N-acetylated-alpha-linked acidic dipeptidase I; NAALADase I; NAALAdase; N-acetylated alpha-linked acidic dipeptidase 1; prostate specific membrane antigen variant F; Prostate-specific membrane antigen; PSMA; PSMAFGCP; PSMPteroylpoly-gamma-glutamate carboxypeptidase

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Citation for Human PSMA/FOLH1/NAALADase I Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Epithelial plasticity and innate immune activation promote lung tissue remodeling following respiratory viral infection
Authors: Beppu, AK;Zhao, J;Yao, C;Carraro, G;Israely, E;Coelho, AL;Drake, K;Hogaboam, CM;Parks, WC;Kolls, JK;Stripp, BR;
Nature communications
Species: Mouse
Sample Types: Organoids
Applications: IHC