Human/Primate VEGF Antibody Summary
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Human/Primate VEGF Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of Recombinant Human VEGF by Western Blot. Western blot shows 25 ng of Recombinant Human VEGF165 (293-VE), Recombinant Human VEGF111 (5336-VE), Recombinant Human VEGF121, aa 207-327 (4644-VS), Recombinant Human VEGF145 (aa 27-171) (7626-VE), Recombinant Human VEGF162 (2347-VE), Recombinant Human VEGF165b (3045-VE), Recombinant Human VEGF189 (aa 27-215) (8147-VE), Recombinant Human VEGF165 Extended Isoform (9018-VE), Recombinant Human VEGF-B167 (751-VE), Recombinant Mouse VEGF164 (493-MV), and Recombinant Rat VEGF164 (564-RV). PVDF Membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Primate VEGF Monoclonal Antibody (Catalog # MAB293) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for VEGF at approximately 15-25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Cell Proliferation Induced by VEGF165and Neutralization by Human VEGF Antibody. Recombinant Human VEGF165 (293-VE) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line) as measured by Resazurin (AR002). Proliferation elicited by Recombinant Human VEGF165 (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Primate VEGF Monoclonal Antibody (Catalog # MAB293). The ND50 is typically 10-60 ng/mL.
Detection of Rat VEGF by Western Blot Protein levels of VEGF (A), KDR (VEGF receptor 2, B), Angiopoietin 1 (C) and Tie2 (D) from retinal endothelial cells cultured in normoxia or hypoxia only and cells in each condition treated with Compound 49b. Hypoxia increased protein levels of all proteins, which were significantly reduced after treatment with Compound 49b. Data is mean ± SEM. *P<0.05 vs. normoxia. #P<0.05 vs. hypoxia. N = 4. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27439004), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Preparation and Storage
Background: VEGF
Vascular Endothelial Growth Factor (VEGF or VEGF-A), also known as Vascular Permeability Factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues and a cystine knot structure. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189. Human VEGF165 is an approximately 44 kDa molecular weight homodimer sharing 88% aa sequence identity with corresponding regions of mouse and rat, 96% with porcine, 95% with canine, and 93% with feline, equine and bovine VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells. Although VEGF affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity. VEGF165 binds the Semaphorin receptor, Neuropilin-1 and promotes complex formation with VEGF R2. VEGF is required during embryogenesis and functions to regulate the proliferation, migration, and survival of endothelial cells. In adults, VEGF functions mainly in wound healing and the female reproductive cycle. Pathologically, it is involved in tumor angiogenesis and vascular leakage. Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus. VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, Oncostatin M (OSM) and TNF-alpha.
Product Datasheets
Citations for Human/Primate VEGF Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Overexpression of Antiangiogenic Vascular Endothelial Growth Factor Isoform and Splicing Regulatory Factors in Oral, Laryngeal and Pharyngeal Squamous Cell Carcinomas
Authors: Patrícia Matos Biselli-Chicote, Joice Matos Biselli, Bianca R Cunha, Rodrigo Castro, José Victor Maniglia, Dalísio de Santi Neto et al.
Asian Pac. J. Cancer Prev
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NFAT5/STAT3 interaction mediates synergism of high salt with IL-17 towards induction of VEGF-A expression in breast cancer cells
Authors: Suneetha Amara, Dalal Alotaibi, Venkataswarup Tiriveedhi
Oncology Letters
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Recombinant Human VEGF165b Inhibits Experimental Choroidal Neovascularization
Authors: Jing Hua, Christine Spee, Satoru Kase, Emma S. Rennel, Anette L. Magnussen, Yan Qiu et al.
Investigative Opthalmology & Visual Science
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Intrathecal B Cells in MS Have Significantly Greater Lymphangiogenic Potential Compared to B Cells Derived From Non-MS Subjects
Authors: Jason Stein, Quangang Xu, Kayla C. Jackson, Elena Romm, Simone C. Wuest, Peter Kosa et al.
Frontiers in Neurology
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Compound 49b Restores Retinal Thickness and Reduces Degenerate Capillaries in the Rat Retina following Ischemia/Reperfusion
PLoS ONE, 2016-07-20;11(7):e0159532.
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Application of a Glucose Dehydrogenase-Fused with Zinc Finger Protein to Label DNA Aptamers for the Electrochemical Detection of VEGF
Authors: Jinhee Lee, Atsuro Tatsumi, Kaori Tsukakoshi, Ellie D. Wilson, Koichi Abe, Koji Sode et al.
Sensors (Basel)
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Small Extracellular Vesicle‐Derived vWF Induces a Positive Feedback Loop between Tumor and Endothelial Cells to Promote Angiogenesis and Metastasis in Hepatocellular Carcinoma
Authors: Samuel Wan Ki Wong, Sze Keong Tey, Xiaowen Mao, Hiu Ling Fung, Zhi‐Jie Xiao, Danny Ka Ho Wong et al.
Advanced Science
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Thymosin ?4 promotes endothelial progenitor cell angiogenesis via a vascular endothelial growth factor?dependent mechanism
Authors: Y Zhao, J Song, X Bi, J Gao, Z Shen, J Zhu, G Fu
Mol Med Rep, 2018-06-20;18(2):2314-2320.
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VEGF −460T → C polymorphism and its association with VEGF expression and outcome to FOLFOX-4 treatment in patients with colorectal carcinoma
Authors: M-H Chen, C-H Tzeng, P-M Chen, J-K Lin, T-C Lin, W-S Chen et al.
The Pharmacogenomics Journal
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Activated androgen receptor accelerates angiogenesis in cutaneous neurofibroma by regulating VEGFA transcription
Authors: Jing Jia, Haibao Zhang, Hongke Zhang, Huicong Du, Wenbo Liu, Maoguo Shu
International Journal of Oncology
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IL-4/IL-13 Stimulated Macrophages Enhance Breast Cancer Invasion Via Rho-GTPase Regulation of Synergistic VEGF/CCL-18 Signaling
Authors: Andrew C. Little, Pragathi Pathanjeli, Zhifen Wu, Liwei Bao, Laura E. Goo, Joel A. Yates et al.
Frontiers in Oncology
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Endocytosis and serpentine filopodia drive blebbishield-mediated resurrection of apoptotic cancer stem cells
Authors: Goodwin G Jinesh, Ashish M Kamat
Cell Death Discovery
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Image-guided genomics of phenotypically heterogeneous populations reveals vascular signalling during symbiotic collective cancer invasion
Authors: J. Konen, E. Summerbell, B. Dwivedi, K. Galior, Y. Hou, L. Rusnak et al.
Nature Communications
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Smad4 inhibits cell migration via suppression of JNK activity in human pancreatic carcinoma PANC-1 cells
Authors: XUEYING ZHANG, JUNXIA CAO, YUJUN PEI, JIYAN ZHANG, QINGYANG WANG
Oncology Letters
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Functional Relevance of the Switch of VEGF Receptors/Co-Receptors during Peritoneal Dialysis-Induced Mesothelial to Mesenchymal Transition
Authors: María Luisa Pérez-Lozano, Pilar Sandoval, Ángela Rynne-Vidal, Abelardo Aguilera, José Antonio Jiménez-Heffernan, Patricia Albar-Vizcaíno et al.
PLoS ONE
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The DEK oncogene activates VEGF expression and promotes tumor angiogenesis and growth in HIF-1 alpha -dependent and -independent manners
Authors: Yanan Zhang, Jie Liu, Shibin Wang, Xiaoli Luo, Yang Li, Zhaohui Lv et al.
Oncotarget
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Diffuse-type gastric carcinoma: progression, angiogenesis, and transforming growth factor beta signaling
Authors: Akiyoshi Komuro, Masakazu Yashiro, Caname Iwata, Yasuyuki Morishita, Erik Johansson, Yoshiko Matsumoto et al.
JNCI: Journal of the National Cancer Institute
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Influence of VEGF-A, VEGFR-1-3, and neuropilin 1-2 on progression-free: and overall survival in WHO grade II and III meningioma patients
Authors: Simon Bernatz, Daniel Monden, Florian Gessler, Tijana Radic, Elke Hattingen, Christian Senft et al.
Journal of Molecular Histology
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NRP-1 interacts with GIPC1 and SYX to activate p38 MAPK signaling and cancer stem cell survival
Authors: Daniel Grun, Gautam Adhikary, Richard L. Eckert
Molecular Carcinogenesis
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Capecitabine metronomic chemotherapy inhibits the proliferation of gastric cancer cells through anti-angiogenesis
Authors: FEI YUAN, HAILONG SHI, JUN JI, QU CAI, XUEHUA CHEN, YINGYAN YU et al.
Oncology Reports
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Upregulation of stromal cell derived factor-1alpha in collagen vascular diseases-associated interstitial pneumonias (CVDs-IPs)
Authors: Giorgos A. Margaritopoulos, Katerina M. Antoniou, Giannoula Soufla, Konstantinos Karagiannis, Athanasia Proklou, Ismini Lasithiotaki et al.
Pulmonary Pharmacology & Therapeutics
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FOXO transcription factors and VEGF neutralizing antibody enhance antiangiogenic effects of resveratrol
Authors: Rakesh K. Srivastava, Terry G. Unterman, Sharmila Shankar
Molecular and Cellular Biochemistry
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Anti-VEGF drug interference with VEGF quantitation in the R&D systems human quantikine VEGF ELISA kit
Authors: Giane Sumner, Camille Georgaros, Ashique Rafique, Thomas DiCioccio, Joel Martin, Nicholas Papadopoulos et al.
Bioanalysis
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Targeting of multiple myeloma-related angiogenesis by miR-199a-5p mimics: in vitro and in vivo anti-tumor activity
Authors: Lavinia Raimondi, Nicola Amodio, Maria Teresa Di Martino, Emanuela Altomare, Marzia Leotta, Daniele Caracciolo et al.
Oncotarget
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The transcription factor GATA1 and the histone methyltransferase SET7 interact to promote VEGF-mediated angiogenesis and tumor growth and predict clinical outcome of breast cancer
Authors: Yanan Zhang, Jie Liu, Jing Lin, Lei Zhou, Yuhua Song, Bo Wei et al.
Oncotarget
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Investigating the paracrine and juxtacrine abilities of adipose-derived stromal cells in angiogenesis triple cell co-cultures
Authors: Harary Søndergaard, R;Drozd Højgaard, L;Haack-Sørensen, M;Hoeeg, C;Mønsted Johansen, E;Follin, B;Kastrup, J;Ekblond, A;Juhl, M;
Stem cell research
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
ADAMTS13 regulates angiogenic markers via Ephrin/Eph signaling in human mesenchymal stem cells under serum-deprivation stress
Authors: Dutta Gupta, S;Ta, M;
Scientific reports
Species: Human
Sample Types: Amino Acid
Applications: Western Blot -
Unravelling heterogeneous effects of cancer?associated fibroblasts on poor prognosis markers in breast cancer EM?G3 cell line: In vitro?targeted treatment (anti?IL-6, anti?VEGF-A, anti?MFGE8) based on transcriptomic profiling
Authors: Urban, L;Novák, ;?oma, M;Dvo?ánková, B;Lacina, L;áchová, J;Hradilová, M;Svato?ová, P;Kolá?, M;Strnad, H;B?ezinová, J;Smetana, K;Gál, P;Szabo, P;
Oncology reports
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
CD40 Ligand-CD40 Interaction Is an Intermediary between Inflammation and Angiogenesis in Proliferative Diabetic Retinopathy
Authors: Abu El-Asrar, AM;Nawaz, MI;Ahmad, A;Dillemans, L;Siddiquei, M;Allegaert, E;Gikandi, PW;De Hertogh, G;Opdenakker, G;Struyf, S;
International journal of molecular sciences
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Effects of Human Deciduous Dental Pulp-Derived Mesenchymal Stem Cell-Derived Conditioned Medium on the Metabolism of HUVECs, Osteoblasts, and BMSCs
Authors: R Kunimatsu, T Hiraki, K Rikitake, K Nakajima, NAR Putranti, T Abe, K Ando, A Nakatani, S Sakata, K Tanimoto
Cells, 2022-10-14;11(20):.
Species: Human
Sample Types: Whole Cells
Applications: ELISA Development, Neutralization -
Fibrocytes boost tumor-supportive phenotypic switches in the lung cancer niche via the endothelin system
Authors: A Weigert, X Zheng, A Nenzel, K Turkowski, S Günther, E Strack, E Sirait-Fis, E Elwakeel, IM Kur, VS Nikam, C Valasaraja, H Winter, A Wissgott, R Voswinkel, F Grimminger, B Brüne, W Seeger, SS Pullamsett, R Savai
Nature Communications, 2022-10-14;13(1):6078.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Inhibition of the VEGF signaling pathway attenuates tumor?associated macrophage activity in liver cancer
Authors: S Okikawa, Y Morine, Y Saito, S Yamada, K Tokuda, H Teraoku, K Miyazaki, S Yamashita, T Ikemoto, S Imura, M Shimada
Oncology reports, 2022-02-16;47(4):.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
Tissue Inhibitor of Metalloproteinase-3 Ameliorates Diabetes-Induced Retinal Inflammation
Authors: AM Abu El-Asr, A Ahmad, MI Nawaz, MM Siddiquei, A De Zutter, L Vanbrabant, PW Gikandi, G Opdenakker, S Struyf
Frontiers in Physiology, 2022-01-10;12(0):807747.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
CD146/Soluble CD146 Pathway Is a Novel Biomarker of Angiogenesis and Inflammation in Proliferative Diabetic Retinopathy
Authors: AM Abu El-Asr, MI Nawaz, A Ahmad, MM Siddiquei, E Allegaert, PW Gikandi, G De Hertogh, G Opdenakker
Investigative Ophthalmology & Visual Science, 2021-07-01;62(9):32.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
3D iPSC modeling of the retinal pigment epithelium-choriocapillaris complex identifies factors involved in the pathology of macular degeneration
Authors: KV Manian, CA Galloway, S Dalvi, AA Emanuel, JA Mereness, W Black, L Winschel, C Soto, Y Li, Y Song, W DeMaria, A Kumar, I Slukvin, MP Schwartz, WL Murphy, B Anand-Apte, M Chung, DSW Benoit, R Singh
Cell Stem Cell, 2021-03-29;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Chrysin Inhibits High Glucose-Induced Migration on Chorioretinal Endothelial Cells via VEGF and VEGFR Down-Regulation
Authors: ZY Liao, IC Liang, HJ Li, CC Wu, HM Lo, DC Chang, CF Hung
Int J Mol Sci, 2020-08-02;21(15):.
Species: Primate, Rhesus Macaque
Sample Types: Cell Lysates
Applications: Western Blot -
Snail promotes the generation of vascular endothelium by breast cancer cells
Authors: Z Chang, Y Zhang, J Liu, Y Zheng, H Li, Y Kong, P Li, H Peng, Y Shi, B Cao, F Ran, Y Chen, Y Song, Q Ye, L Ding
Cell Death Dis, 2020-06-15;11(6):457.
Species: Human
Sample Types: Transduced Whole Cells
Applications: Neutralization -
Migration Inhibitory Factor in Conditioned Medium from Human Umbilical Cord Blood-Derived Mesenchymal Stromal Cells Stimulates Hair Growth
Authors: HA Oh, J Kwak, BJ Kim, HJ Jin, WS Park, SJ Choi, W Oh, S Um
Cells, 2020-05-28;9(6):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Quercetin attenuates adipose hypertrophy, in part through activation of adipogenesis in rats fed a high-fat diet
Authors: DJ Perdicaro, C Rodriguez, J Gambarte T, RM Miatello, PI Oteiza, MA Vazquez Pr
J. Nutr. Biochem., 2020-02-04;79(0):108352.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
Contribution of the VEGF system to the follicular persistence associated with bovine cystic ovaries
Authors: AF Stassi, F Gasser, MML Velázquez, EM Belotti, NC Gareis, F Rey, HH Ortega, NR Salvetti, ME Baravalle
Theriogenology, 2019-07-03;138(0):52-65.
Species: Bovine, Mouse
Sample Types: Cell Lysates, Tissue Homogenates, Whole Tissue
Applications: IHC, IHC-P, Western Blot -
TSP-1 is downregulated and inversely correlates with miR-449c expression in Cushing's disease
Authors: J Ren, C Gu, Y Yang, J Xue, Y Sun, F Jian, D Chen, L Bian, Q Sun
J. Cell. Mol. Med., 2019-04-23;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
TRF2 positively regulates SULF2 expression increasing VEGF-A release and activity in tumor microenvironment
Authors: P Zizza, R Dinami, M Porru, C Cingolani, E Salvati, A Rizzo, C D'Angelo, E Petti, CA Amoreo, M Mottolese, I Sperduti, A Chambery, R Russo, P Ostano, G Chiorino, G Blandino, A Sacconi, J Cherfils-V, C Leonetti, E Gilson, A Biroccio
Nucleic Acids Res., 2019-04-23;0(0):.
Species: Human, Mouse
Sample Types: In Vivo, Whole Cells
Applications: Neutralization -
A Subset of Paracrine Factors as Efficient Biomarkers for Predicting Vascular Regenerative Efficacy of Mesenchymal Stromal/Stem Cells
Authors: HK Kim, SG Lee, SW Lee, BJ Oh, JH Kim, JA Kim, G Lee, JD Jang, YA Joe
Stem Cells, 2018-10-08;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Soluble E-cadherin promotes tumor angiogenesis and localizes to exosome surface
Authors: MKS Tang, PYK Yue, PP Ip, RL Huang, HC Lai, ANY Cheung, KY Tse, HYS Ngan, AST Wong
Nat Commun, 2018-06-11;9(1):2270.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Grape pomace extract induced beige cells in white adipose tissue from rats and in 3T3-L1 adipocytes
Authors: C Rodriguez, DJ Perdicaro, MS Landa, A Fontana, A Antoniolli, RM Miatello, PI Oteiza, MA Vazquez Pr
J. Nutr. Biochem., 2018-03-10;56(0):224-233.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
Targeting angiogenesis in multiple myeloma by the VEGF and HGF blocking DARPin� protein MP0250: a preclinical study
Authors: L Rao, K De Veirman, D Giannico, I Saltarella, V Desantis, MA Frassanito, AG Solimando, D Ribatti, M Prete, A Harstrick, U Fiedler, H De Raeve, V Racanelli, K Vanderkerk, A Vacca
Oncotarget, 2018-01-30;9(17):13366-13381.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Investigating Colorimetric Protein Array Assay Schemes for Detection of Recurrence of Bladder Cancer
Authors: S Gogalic, U Sauer, S Doppler, C Preininger
Biosensors (Basel), 2018-01-24;8(1):.
Species: Human
Sample Types: Urine
Applications: Array Development -
Application of an acellular dermal matrix to a rabbit model of oral mucosal defects
Authors: X Xu, N Cui, E Wang
Exp Ther Med, 2018-01-05;15(3):2450-2456.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
Predictive Value of Clinical Findings and Plasma Biomarkers after Fourteen Days of Prednisone Treatment for Acute Graft-versus-host Disease.
Authors: McDonald G, Tabellini L, Storer B, Martin P, Lawler R, Rosinski S, Schoch H, Hansen J
Biol Blood Marrow Transplant, 2017-05-03;23(8):1257-1263.
Species: Human
Sample Types: Plasma
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HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia
Authors: P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu
Oncotarget, 2017-04-25;8(17):28074-28092.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Vessel morphometric parameters-correlation with histologic grade and VEGF expression in oligodendroglioma
Authors: LB Strickland, S Brem, AM Rojiani, MV Rojiani
Am J Cancer Res, 2017-04-01;7(4):973-981.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Angiogenesis in newly regenerated bone by secretomes of human mesenchymal stem cells
Authors: W Katagiri, T Kawai, M Osugi, Y Sugimura-W, K Sakaguchi, T Kojima, T Kobayashi
Maxillofac Plast Reconstr Surg, 2017-03-25;39(1):8.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Development of a surrogate potency assay to determine the angiogenic activity of Stempeucel�, a pooled, ex-vivo expanded, allogeneic human bone marrow mesenchymal stromal cell product
Authors: C Thej, B Ramadasse, A Walvekar, AS Majumdar, S Balasubram
Stem Cell Res Ther, 2017-02-28;8(1):47.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Transendothelial migration of human umbilical mesenchymal stem cells across uterine endothelial monolayers: Junctional dynamics and putative mechanisms
Authors: Lopa Leach
Placenta, 2016-10-21;48(0):87-98.
Species: Human
Sample Types: Whole Cells
Applications: ICC, Neutralization -
The Proton-Sensing G-Protein Coupled Receptor GPR4 Promotes Angiogenesis in Head and Neck Cancer
Authors: Z Jing, H Xu, X Chen, Q Zhong, J Huang, Y Zhang, W Guo, Z Yang, S Ding, P Chen, Z Huang
PLoS ONE, 2016-04-14;11(4):e0152789.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
VEGF-A acts via neuropilin-1 to enhance epidermal cancer stem cell survival and formation of aggressive and highly vascularized tumors
Authors: D Grun, G Adhikary, RL Eckert
Oncogene, 2016-01-25;35(33):4379-87.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Interleukin-1 Receptor Type 2 Acts with c-Fos to Enhance the Expression of Interleukin-6 and Vascular Endothelial Growth Factor A in Colon Cancer Cells and Induce Angiogenesis.
Authors: Mar A, Chu C, Lee H, Chien C, Cheng J, Yang S, Jiang J, Lee T
J Biol Chem, 2015-07-24;290(36):22212-24.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Pericytes contribute to the disruption of the cerebral endothelial barrier via increasing VEGF expression: implications for stroke.
Authors: Bai Y, Zhu X, Chao J, Zhang Y, Qian C, Li P, Liu D, Han B, Zhao L, Zhang J, Buch S, Teng G, Hu G, Yao H
PLoS ONE, 2015-04-17;10(4):e0124362.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Survivin expression promotes VEGF-induced tumor angiogenesis via PI3K/Akt enhanced beta-catenin/Tcf-Lef dependent transcription.
Authors: Fernandez J, Rodriguez D, Valenzuela M, Calderon C, Urzua U, Munroe D, Rosas C, Lemus D, Diaz N, Wright M, Leyton L, Tapia J, Quest A
Mol Cancer, 2014-09-09;13(0):209.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Breast cancer cells condition lymphatic endothelial cells within pre-metastatic niches to promote metastasis.
Authors: Lee E, Fertig E, Jin K, Sukumar S, Pandey N, Popel A
Nat Commun, 2014-09-02;5(0):4715.
Species: Human
Sample Types: In Vivo, Whole Tissue
Applications: IHC, Neutralization -
Interleukin 4, interleukin 6 and osteopontin-serological markers of head and neck malignancy in primary diagnostics: A pilot study.
Authors: Aderhold C, Grobschmidt G, Sauter A, Faber A, Hormann K, Schultz J
Oncol Lett, 2014-07-04;8(3):1112-1118.
Species: Human
Sample Types: Serum
Applications: Bioassay -
Control of angiogenesis by galectins involves the release of platelet-derived proangiogenic factors.
Authors: Etulain J, Negrotto S, Tribulatti M, Croci D, Carabelli J, Campetella O, Rabinovich G, Schattner M
PLoS ONE, 2014-04-30;9(4):e96402.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Enhanced efficacy of combination therapy with adenoassociated virus-delivered pigment epithelium-derived factor and cisplatin in a mouse model of Lewis lung carcinoma.
Authors: He S, Wu Q, Gong C, Luo S, Zhang S, Li M, Lu L, Wei Y, Yang L
Mol Med Rep, 2014-04-04;9(6):2069-76.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Hematopoietic stem cell cytokines and fibroblast growth factor-2 stimulate human endothelial cell-pericyte tube co-assembly in 3D fibrin matrices under serum-free defined conditions.
Authors: Smith A, Bowers S, Stratman A, Davis G
PLoS ONE, 2013-12-31;8(12):e85147.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Markers of T cell infiltration and function associate with favorable outcome in vascularized high-grade serous ovarian carcinoma.
Authors: Townsend, Katelin, Spowart, Jaeline, Huwait, Hassan, Eshragh, Sima, West, Nathan R, Elrick, Mary A, Kalloger, Steve E, Anglesio, Michael, Watson, Peter H, Huntsman, David G, Lum, Julian J
PLoS ONE, 2013-12-23;8(12):e82406.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Heat shock protein 27 attenuates neointima formation and accelerates reendothelialization after arterial injury and stent implantation: importance of vascular endothelial growth factor up-regulation.
Authors: Ma X, Hibbert B, McNulty M, Hu T, Zhao X, Ramirez F, Simard T, de Belleroche J, O'Brien E
FASEB J, 2013-10-18;28(2):594-602.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
High-density lipoproteins augment hypoxia-induced angiogenesis via regulation of post-translational modulation of hypoxia-inducible factor 1alpha.
Authors: Tan J, Prosser H, Vanags L, Monger S, Ng M, Bursill C
FASEB J, 2013-09-10;28(1):206-17.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
The transcription factor E2F1 and the SR protein SC35 control the ratio of pro-angiogenic versus antiangiogenic isoforms of vascular endothelial growth factor-A to inhibit neovascularization in vivo.
Authors: Merdzhanova G, Gout S, Keramidas M, Edmond V, Coll JL, Brambilla C, Brambilla E, Gazzeri S, Eymin B
Oncogene, 2010-07-19;29(39):5392-403.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
VEGF in the lung: a role for novel isoforms.
Authors: Varet J, Varet, Julia, Douglas SK, Douglas, Samantha, Gilmartin L, Gilmartin, Laura, Medford, Andrew R, Bates, David O, Harper, Steven J, Millar, Ann B
Am J Physiol Lung Cell Mol Physiol, 2010-03-12;298(6):L768-74.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
Complementary actions of inhibitors of angiopoietin-2 and VEGF on tumor angiogenesis and growth.
Authors: Hashizume H, Falcon BL, Kuroda T, Baluk P, Coxon A, Yu D, Bready JV, Oliner JD, McDonald DM
Cancer Res., 2010-03-02;70(6):2213-23.
Species: Human
Sample Types: In Vivo
Applications: Neutralization -
A small-molecule triptolide suppresses angiogenesis and invasion of human anaplastic thyroid carcinoma cells via down-regulation of the nuclear factor-kappa B pathway.
Authors: Zhu W, Ou Y, Li Y, Xiao R, Shu M, Zhou Y, Xie J, He S, Qiu P, Yan G
Mol. Pharmacol., 2009-01-21;75(4):812-9.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Development and validation of sandwich ELISA microarrays with minimal assay interference.
Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
J. Proteome Res., 2008-04-19;7(6):2406-14.
Species: Human
Sample Types: Serum
Applications: ELISA Microarray Development -
FGF5 as an oncogenic factor in human glioblastoma multiforme: autocrine and paracrine activities.
Authors: Allerstorfer S, Sonvilla G, Fischer H, Spiegl-Kreinecker S, Gauglhofer C, Setinek U, Czech T, Marosi C, Buchroithner J, Pichler J, Silye R, Mohr T, Holzmann K, Grasl-Kraupp B, Marian B, Grusch M, Fischer J, Micksche M, Berger W
Oncogene, 2008-03-24;27(30):4180-90.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Ultrasensitive flow-based immunoassays using single-molecule counting.
Authors: Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
Clin. Chem., 2007-09-21;53(11):1990-5.
Species: Human
Sample Types: Plasma
Applications: ELISA Development -
Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.
Authors: Ball SG, Shuttleworth CA, Kielty CM
J. Cell Biol., 2007-04-30;177(3):489-500.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Microvascular endothelial cells increase proliferation and inhibit apoptosis of native human acute myelogenous leukemia blasts.
Authors: Hatfield K, Ryningen A, Corbascio M, Bruserud O
Int. J. Cancer, 2006-11-15;119(10):2313-21.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Placenta growth factor in diabetic wound healing: altered expression and therapeutic potential.
Authors: Cianfarani F, Zambruno G, Brogelli L, Sera F, Lacal PM, Pesce M, Capogrossi MC, Failla CM, Napolitano M, Odorisio T
Am. J. Pathol., 2006-10-01;169(4):1167-82.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Vascular endothelial growth factor-A induces plaque expansion in ApoE knock-out mice by promoting de novo leukocyte recruitment.
Authors: Lucerna M, Zernecke A, de Nooijer R, de Jager SC, Bot I, van der Lans C, Kholova I, Liehn EA, van Berkel TJ, Yla-Herttuala S, Weber C, Biessen EA
Blood, 2006-09-21;109(1):122-9.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Neutralization -
Acidic extracellular pH promotes experimental metastasis of human melanoma cells in athymic nude mice.
Authors: Rofstad EK, Mathiesen B, Kindem K, Galappathi K
Cancer Res., 2006-07-01;66(13):6699-707.
Species: Complex Species Category
Sample Types: In Vivo
Applications: Neutralization -
The endogenous anti-angiogenic family of splice variants of VEGF, VEGFxxxb, are down-regulated in pre-eclamptic placentae at term.
Authors: Bates DO, MacMillan PP, Manjaly JG, Qiu Y, Hudson SJ, Bevan HS, Hunter AJ, Soothill PW, Read M, Donaldson LF, Harper SJ
Clin. Sci., 2006-05-01;110(5):575-85.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
Increased vascular endothelial growth factor and receptors: relationship to angiogenesis in asthma.
Authors: Feltis BN, Wignarajah D, Zheng L, Ward C, Reid D, Harding R, Walters EH
Am. J. Respir. Crit. Care Med., 2006-03-09;173(11):1201-7.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
J. Immunol., 2005-11-01;175(9):6177-89.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Neutralization -
Functional and structural remodeling of the myocardial microvasculature in early experimental hypertension.
Authors: Rodriguez-Porcel M, Zhu XY, Chade AR, Amores-Arriaga B, Caplice NM, Ritman EL, Lerman A, Lerman LO
Am. J. Physiol. Heart Circ. Physiol., 2005-10-07;290(3):H978-84.
Species: Porcine
Sample Types: Whole Tissue
Applications: IHC-Fr -
Platelet-derived growth factor-AA is an essential and autocrine regulator of vascular endothelial growth factor expression in non-small cell lung carcinomas.
Authors: Shikada Y, Yonemitsu Y, Koga T, Onimaru M, Nakano T, Okano S, Sata S, Nakagawa K, Yoshino I, Maehara Y, Sueishi K
Cancer Res., 2005-08-15;65(16):7241-8.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Evaluating hypoxia-inducible factor-1alpha as a cancer therapeutic target via inducible RNA interference in vivo.
Authors: Li L, Lin X, Staver M, Shoemaker A, Semizarov D, Fesik SW, Shen Y
Cancer Res., 2005-08-15;65(16):7249-58.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Possible mechanisms inducing glomerulations in interstitial cystitis: relationship between endoscopic findings and expression of angiogenic growth factors.
Authors: Tamaki M, Saito R, Ogawa O, Yoshimura N, Ueda T
J. Urol., 2004-09-01;172(3):945-8.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Plasma angiopoietin-1, angiopoietin-2 and Tie-2 in breast and prostate cancer: a comparison with VEGF and Flt-1.
Authors: Caine GJ, Blann AD, Stonelake PS, Ryan P, Lip GY
Eur. J. Clin. Invest., 2003-10-01;33(10):883-90.
Species: Human
Sample Types: Plasma
Applications: ELISA Development -
Expression of the CD44v2-10 isoform confers a metastatic phenotype: importance of the heparan sulfate attachment site CD44v3.
Authors: Barbour AP, Reeder JA, Walsh MD, Fawcett J, Antalis TM, Gotley DC
Cancer Res., 2003-02-15;63(4):887-92.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Vascular endothelial growth factor ligands and receptors that regulate human cytotrophoblast survival are dysregulated in severe preeclampsia and hemolysis, elevated liver enzymes, and low platelets syndrome.
Authors: Zhou Y, 2019, McMaster M, Woo K, Janatpour M, Perry J, Karpanen T, Alitalo K, Damsky C, Fisher SJ
2002-04-01;160(4):1405-23.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Hepatocyte growth factor and vascular endothelial growth factor expression in pig-to-primate xenotransplanted hearts.
Authors: Takayama K, Suzuki J, Kawauchi M, Tsukioka K, Wada Y, Zhang T, Endoh M, Takamoto S, Amano J, Isobe M
Transplant. Proc., 2000-08-01;32(5):987-9.
Species: Porcine
Sample Types: Whole Tissue
Applications: IHC-P -
Inhibition of Semaphorin As a Novel Strategy for Therapeutic Angiogenesis
Authors: Junji Moriya, Tohru Minamino, Kaoru Tateno, Sho Okada, Akiyoshi Uemura, Ippei Shimizu et al.
Circulation Research
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Ionizing Radiation-Induced GDF15 Promotes Angiogenesis in Human Glioblastoma Models by Promoting VEGFA Expression Through p-MAPK1/SP1 Signaling
Authors: Hyejin Park, Ki-Seok Nam, Hae-June Lee, Kwang Seok Kim
Frontiers in Oncology
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Induction of Angiogenesis by Genetically Modified Human Umbilical Cord Blood Mononuclear Cells
Authors: Dilara Z. Gatina, Ilnaz M. Gazizov, Margarita N. Zhuravleva, Svetlana S. Arkhipova, Maria A. Golubenko, Marina O. Gomzikova et al.
International Journal of Molecular Sciences
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Interleukin-6 as a potential molecular target in esophageal squamous cell carcinoma
Authors: ZHI-FEI ZHAO, JIAN-XIONG LI, RUI YE, XUAN WU, LING-LING GAO, BAO-LONG NIU
Oncology Letters
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A robust automated measure of average antibody staining in immunohistochemistry images.
Authors: Choudhury KR, Yagle KJ, Swanson PE et al.
J Histochem Cytochem
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Anti-human Vascular Endothelial Growth Factor (VEGF) Antibody Selection for Immunohistochemical Staining of Proliferating Blood Vessels
Authors: Chris M. van der Loos, Lorine B. Meijer-Jorna, Marloes E.C. Broekmans, Hanneke P.H.M. Ploegmakers, Peter Teeling, Onno J. de Boer et al.
Journal of Histochemistry & Cytochemistry
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Hypoxia-induced expression of VEGF is reversible in myocardial vascular smooth muscle cells.
Authors: Gu JW, Adair TH
Am. J. Physiol., 1997-08-01;273(2):H628-33.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Neutralization -
The Proinflammatory and Proangiogenic Macrophage Migration Inhibitory Factor Is a Potential Regulator in Proliferative Diabetic Retinopathy
Authors: Ahmed M. Abu El-Asrar, Ajmal Ahmad, Mohammad Mairaj Siddiquei, Alexandra De Zutter, Eef Allegaert, Priscilla W. Gikandi et al.
Frontiers in Immunology
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Contrast-Enhanced Ultrasonography Features of Breast Malignancies with Different Sizes: Correlation with Prognostic Factors
Authors: Li-Xia Zhao, Hui Liu, Qing Wei, Guang Xu, Jian Wu, Hui-Xiong Xu et al.
BioMed Research International
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Tie2/TEK Modulates the Interaction of Glioma and Brain Tumor Stem Cells with Endothelial Cells and Promotes an Invasive Phenotype
Authors: Dan Liu, Vanesa Martin, Juan Fueyo, Ok-Hee Lee, Jing Xu, Nahir Cortes-Santiago et al.
ImpactJ
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Beyond proliferation: KLF5 promotes angiogenesis of bladder cancer through directly regulating VEGFA transcription
Authors: Yang Gao, Kaijie Wu, Yule Chen, Jiancheng Zhou, Chong Du, Qi Shi et al.
Oncotarget
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DCE-MRI in Glioma, Infiltration Zone and Healthy Brain to Assess Angiogenesis: A Biopsy Study
Authors: Vera C. Keil, Gerrit H. Gielen, Bogdan Pintea, Peter Baumgarten, Angeliki Datsi, Kanishka Hittatiya et al.
Clinical Neuroradiology
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Upregulation of Thrombin/Matrix Metalloproteinase-1/Protease-Activated Receptor-1 Chain in Proliferative Diabetic Retinopathy
Authors: Ahmed M. Abu El-Asrar, Kaiser Alam, Mohd Imtiaz Nawaz, Ghulam Mohammad, Kathleen Van den Eynde, Mohammad Mairaj Siddiquei et al.
Current Eye Research
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Dimethyloxalyglycine stimulates the early stages of gastrointestinal repair processes through VEGF-dependent mechanisms
Authors: Tania Marchbank, Asif Mahmood, Sarah Harten, Patrick H Maxwell, Raymond J Playford
Laboratory Investigation
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Identification of novel neutralizing single-chain antibodies against vascular endothelial growth factor receptor 2
Authors: Berrin Erdag, B Koray Koray Balcioglu, Aylin Ozdemir Ozdemir Bahadir, Muge Serhatli, Omer Kacar, Aydin Bahar et al.
Biotechnology and Applied Biochemistry
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Cancer-derived small extracellular vesicles promote angiogenesis by heparin-bound, bevacizumab-insensitive VEGF, independent of vesicle uptake
Authors: Song Yi Ko, WonJae Lee, Hilary A. Kenny, Long H. Dang, Lee M. Ellis, Eric Jonasch et al.
Communications Biology
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VEGF, FLT3 ligand, PlGF and HGF can substitute for M-CSF to induce human osteoclast formation: implications for giant cell tumour pathobiology
Authors: Richard M Taylor, Takeshi G Kashima, Helen J Knowles, Nicholas A Athanasou
Laboratory Investigation
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B7-H3 promotes colorectal cancer angiogenesis through activating the NF-kappa B pathway to induce VEGFA expression
Authors: Ruoqin Wang, Yanchao Ma, Shenghua Zhan, Guangbo Zhang, Lei Cao, Xueguang Zhang et al.
Cell Death & Disease
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Roundabout4 Suppresses Glioma-Induced Endothelial Cell Proliferation, Migration and Tube Formation in Vitro by Inhibiting VEGR2-Mediated PI3K/AKT and FAK Signaling Pathways
Authors: Heng Cai, Yixue Xue, Zhen Li, Yi Hu, Zhenhua Wang, Wenjing Liu et al.
Cellular Physiology and Biochemistry
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Evaluation of Proteoforms of the Transmembrane Chemokines CXCL16 and CX3CL1, Their Receptors, and Their Processing Metalloproteinases ADAM10 and ADAM17 in Proliferative Diabetic Retinopathy
Authors: Ahmed M. Abu El-Asrar, Mohd Imtiaz Nawaz, Ajmal Ahmad, Alexandra De Zutter, Mohammad Mairaj Siddiquei, Marfa Blanter et al.
Frontiers in Immunology
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Strong Expression of Hypoxia-Inducible Factor-1alpha (HIF-1alpha) Is Associated with Axl Expression and Features of Aggressive Tumors in African Breast Cancer.
Authors: Nalwoga H, Ahmed L, Arnes J, Wabinga H, Akslen L
PLoS ONE, 2016-01-13;11(1):e0146823.
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Platelet-derived adenosine 5′-triphosphate suppresses activation of human hepatic stellate cell: In vitro study
Authors: Naoya Ikeda, Soichiro Murata, Takehito Maruyama, Takafumi Tamura, Reiji Nozaki, Takuya Kawasaki et al.
Hepatology Research
FAQs
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What are the differences between MAB293 and MAB293R?
MAB293 is produced by culturing a hybridoma cell line, whereas MAB293R is produced via recombinant DNA technology, based on the known, proprietary sequence of MAB293. The benefit of recombinant antibodies is the ability to offer a more consistent antibody, with an indefinite supply. As far as research applications, both products will work the same.
Reviews for Human/Primate VEGF Antibody
Average Rating: 4.8 (Based on 8 Reviews)
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IF of HUVEC cells with exogenous VEGF internalized (red) and colocalizing with VEGF receptor (green)
We used this VEGF-A neutralizing antibody (10 ng/mL) as part of an antibody cocktail containing several other antibodies to neutralize VEGF-A present in the Conditioned Medium (CM) from BT549 Cells treated with Angiotensin II CM (+/- VEGF-A Neutralizing ab Cocktail) was used to further look at Endothelial (HUVEC) Cell migration using Incucyte Chemotaxis Assay.
Cancer Res; 78(5) March 1, 2018 (Fig 6F)
After biotinylation, used as a capture reagent in MSD assay to detect ocular VEGF level in a swine model of laser-induced choroidal neovascularization. A standard curve with pig VEGF from Bio-Rad (Cat# PPP030) is shown (2-8,000 pg/ml).