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Human/Primate VEGF165 Biotinylated Antibody

Catalog #: BAF293
28 Citations 1 Review Datasheet / COA / SDS
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Detection of Rat VEGF by Western Blot
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Citations (28)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Primate VEGF165 Biotinylated Antibody Summary

Species Reactivity
Human, Primate
Specificity
Detects human VEGF165 in ELISAs and Western blots. In sandwich immunoassays, less than 15% cross-reactivity with recombinant human (rh) VEGF/PlGF is observed and less than 0.1% cross-reactivity with recombinant mouse (rm) PlGF-2, rhFlt-1, rhVEGF R2, rhPlGF, rhVEGF‑C, rhVEGF-D, and rmVEGF is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF165 (R&D Systems, Catalog # 293-VE)
Ala27-Arg191
Accession # AAV38412
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human VEGF165 (Catalog # 293-VE)

Human/Primate VEGF Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human/Primate VEGF Antibody (Catalog # MAB293R)

Capture Reagent: Human/Primate VEGF Antibody (Catalog # MAB293)

Standard: Recombinant Human VEGF 165 Protein (Catalog # 293-VE)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Rat VEGF by Western Blot View Larger

Detection of Rat VEGF by Western Blot Protein levels of VEGF (A), KDR (VEGF receptor 2, B), Angiopoietin 1 (C) and Tie2 (D) from retinal endothelial cells cultured in normoxia or hypoxia only and cells in each condition treated with Compound 49b. Hypoxia increased protein levels of all proteins, which were significantly reduced after treatment with Compound 49b. Data is mean ± SEM. *P<0.05 vs. normoxia. #P<0.05 vs. hypoxia. N = 4. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27439004), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGF

Vascular Endothelial Growth Factor (VEGF or VEGF-A), also known as Vascular Permeability Factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues and a cystine knot structure. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189. Human VEGF165 is an approximately 44 kDa molecular weight homodimer sharing 88% aa sequence identity with corresponding regions of mouse and rat, 96% with porcine, 95% with canine, and 93% with feline, equine and bovine VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells. Although VEGF affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity. VEGF165 binds the Semaphorin receptor, Neuropilin-1 and promotes complex formation with VEGF R2. VEGF is required during embryogenesis and functions to regulate the proliferation, migration, and survival of endothelial cells. In adults, VEGF functions mainly in wound healing and the female reproductive cycle. Pathologically, it is involved in tumor angiogenesis and vascular leakage. Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus. VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, Oncostatin M (OSM) and TNF-alpha.

Long Name
Vascular Endothelial Growth Factor
Entrez Gene IDs
7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)
Alternate Names
MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor

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Citations for Human/Primate VEGF165 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

28 Citations: Showing 1 - 10
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  1. Application of a Glucose Dehydrogenase-Fused with Zinc Finger Protein to Label DNA Aptamers for the Electrochemical Detection of VEGF
    Authors: Jinhee Lee, Atsuro Tatsumi, Kaori Tsukakoshi, Ellie D. Wilson, Koichi Abe, Koji Sode et al.
    Sensors (Basel)
  2. Vascular endothelial growth factor isoforms differentially protect neurons against neurotoxic events associated with Alzheimer’s disease
    Authors: Roaa H. Alalwany, Tom Hawtrey, Kevin Morgan, Jonathan C. Morris, Lucy F. Donaldson, David O. Bates
    Frontiers in Molecular Neuroscience
  3. Suppression of vascular endothelial growth factor expression at the transcriptional and post-transcriptional levels
    Authors: Heung-Sun Kwon, Hyun-Chul Shin, Jin-Soo Kim
    Nucleic Acids Research
  4. Canine Bone Marrow Derived Mesenchymal Stromal Cells Suppress Allo-Reactive Lymphocyte Proliferation in Vitro but Fail to Enhance Engraftment in Canine Bone Marrow Transplantation
    Authors: Won Sik Lee, Yasuhiro Suzuki, Scott S. Graves, Mineo Iwata, G.M. Venkataraman, Marco Mielcarek et al.
    Biology of Blood and Marrow Transplantation
  5. Amperometric Biosensor for Quantitative Measurement Using Sandwich Immunoassays
    Authors: Pedersen, T;Fojan, P;Pedersen, AKN;Magnusson, NE;Gurevich, L;
    Biosensors
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Detection
  6. Central nervous system hemangioblastomas in von Hippel-Lindau disease: Total growth rate and risk of developing new lesions not associated with circulating VEGF levels
    Authors: J Sundblom, TP Skare, O Holm, S Welin, M Braun, P Nilsson, P Enblad, EO Sjöström, A Smits
    PLoS ONE, 2022-11-28;17(11):e0278166.
    Species: Human
    Sample Types: Plasma
    Applications: PLA
  7. TIMP3/TGF?&beta1 axis regulates mechanical loading?induced chondrocyte degeneration and angiogenesis
    Authors: DL Zhao, HT Li, SH Liu
    Molecular Medicine Reports, 2020-07-29;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  8. Multiple Sclerosis Treatment and Melanoma Development
    Authors: ML Carbone, PM Lacal, S Messinese, L De Giglio, C Pozzilli, S Persechino, C Mazzanti, CM Failla, G Pagnanelli
    Int J Mol Sci, 2020-04-22;21(8):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ELISA Development (Detection)
  9. Endothelialization of arterial vascular grafts by circulating monocytes
    Authors: RJ Smith, B Nasiri, J Kann, D Yergeau, JE Bard, DD Swartz, ST Andreadis
    Nat Commun, 2020-04-01;11(1):1622.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. SRPK1 maintains acute myeloid leukemia through effects on isoform usage of epigenetic regulators including BRD4
    Authors: K Tzelepis, E De Braekel, D Aspris, I Barbieri, MS Vijayabask, WH Liu, M Gozdecka, E Metzakopia, HD Toop, M Dudek, SC Robson, F Hermida-Pr, YH Yang, R Babaei-Jad, DA Garyfallos, H Ponstingl, JML Dias, P Gallipoli, M Seiler, S Buonamici, B Vick, AJ Bannister, R Rad, RK Prinjha, JC Marioni, B Huntly, J Batson, JC Morris, C Pina, A Bradley, I Jeremias, DO Bates, K Yusa, T Kouzarides, GS Vassiliou
    Nat Commun, 2018-12-19;9(1):5378.
    Species: Human
    Sample Types: Cell Lysates
  11. Investigating Colorimetric Protein Array Assay Schemes for Detection of Recurrence of Bladder Cancer
    Authors: S Gogalic, U Sauer, S Doppler, C Preininger
    Biosensors (Basel), 2018-01-24;8(1):.
    Species: Human
    Sample Types: Urine
    Applications: Array Development
  12. Reduced microRNA-503 expression augments lung fibroblast VEGF production in chronic obstructive pulmonary disease
    Authors: J Ikari, AJ Nelson, J Obaid, A Giron-Mart, K Ikari, F Makino, S Iwasawa, Y Gunji, M Farid, X Wang, H Basma, D Demeo, C Feghali-Bo, O Holz, K Rabe, X Liu, SI Rennard
    PLoS ONE, 2017-09-07;12(9):e0184039.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  13. The anti-vascular endothelial growth factor receptor-1 monoclonal antibody D16F7 inhibits invasiveness of human glioblastoma and glioblastoma stem cells
    Authors: MG Atzori, L Tentori, F Ruffini, C Ceci, L Lisi, E Bonanno, M Scimeca, E Eskilsson, T Daubon, H Miletic, L Ricci Viti, R Pallini, P Navarra, R Bjerkvig, S D'Atri, PM Lacal, G Graziani
    J. Exp. Clin. Cancer Res., 2017-08-10;36(1):106.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  14. Predictive Value of Clinical Findings and Plasma Biomarkers after Fourteen Days of Prednisone Treatment for Acute Graft-versus-host Disease.
    Authors: McDonald G, Tabellini L, Storer B, Martin P, Lawler R, Rosinski S, Schoch H, Hansen J
    Biol Blood Marrow Transplant, 2017-05-03;23(8):1257-1263.
    Species: Human
    Sample Types: Plasma
  15. Interleukin-10 and prostaglandin E2 have complementary but distinct suppressive effects on Toll-like receptor-mediated dendritic cell activation in ovarian carcinoma
    Authors: E Brencicova, AL Jagger, HG Evans, M Georgouli, A Laios, S Attard Mon, G Mehra, J Spencer, AA Ahmed, S Raju-Kanki, LS Taams, SS Diebold
    PLoS ONE, 2017-04-14;12(4):e0175712.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  16. Ellagic Acid Inhibits Bladder Cancer Invasiveness and In Vivo Tumor Growth
    Authors: Claudia Ceci
    Nutrients, 2016-11-22;8(11):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  17. Compound 49b Restores Retinal Thickness and Reduces Degenerate Capillaries in the Rat Retina following Ischemia/Reperfusion
    PLoS ONE, 2016-07-20;11(7):e0159532.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. Granzyme B releases vascular endothelial growth factor from extracellular matrix and induces vascular permeability.
    Authors: Hendel, Alon, Hsu, Ivy, Granville, David J
    Lab Invest, 2014-05-05;94(7):716-25.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  19. Platelet-derived growth factor C and calpain-3 are modulators of human melanoma cell invasiveness.
    Authors: Ruffini F, Tentori L, Dorio A, Arcelli D, D'Amati G, D'Atri S, Graziani G, Lacal P
    Oncol Rep, 2013-10-10;30(6):2887-96.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  20. Vascular endothelial growth factor signals through platelet-derived growth factor receptor beta in meningiomas in vitro.
    Br. J. Cancer, 2012-10-09;107(10):1702-13.
    Species: Human
    Sample Types: Cell Lysates
    Applications: TaqMan Protein Assay
  21. Sensitive and multiplexed detection of proteomic antigens via quantum dot aggregation.
    Authors: Soman C, Giorgio T
    Nanomedicine, 2009-02-13;5(4):402-9.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Quantum Dot
  22. Development and validation of sandwich ELISA microarrays with minimal assay interference.
    Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
    J. Proteome Res., 2008-04-19;7(6):2406-14.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Microarray Development
  23. Ultrasensitive flow-based immunoassays using single-molecule counting.
    Authors: Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
    Clin. Chem., 2007-09-21;53(11):1990-5.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  24. Marked differences in human melanoma antigen-specific T cell responsiveness after vaccination using a functional microarray.
    Authors: Chen DS, Soen Y, Stuge TB
    PLoS Med., 2005-09-20;2(10):e265.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  25. Plasma angiopoietin-1, angiopoietin-2 and Tie-2 in breast and prostate cancer: a comparison with VEGF and Flt-1.
    Authors: Caine GJ, Blann AD, Stonelake PS, Ryan P, Lip GY
    Eur. J. Clin. Invest., 2003-10-01;33(10):883-90.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  26. Imbalance in the expression of CXC chemokines correlates with bronchoalveolar lavage fluid angiogenic activity and procollagen levels in acute respiratory distress syndrome.
    Authors: Keane MP, Donnelly SC, Belperio JA, Goodman RB, Dy M, Burdick MD, Fishbein MC, Strieter RM
    J. Immunol., 2002-12-01;169(11):6515-21.
    Species: Human
    Sample Types: BALF
    Applications: ELISA Development
  27. The carboxyl terminus of VEGF-A is a potential target for anti-angiogenic therapy
    Authors: James G. Carter, Melissa V. R. Gammons, Gopinath Damodaran, Amanda J. Churchill, Steven J. Harper, David O. Bates
    Angiogenesis
  28. Isolation and Properties of an In Vitro Human Outer Blood-Retinal Barrier Model
    Authors: Robin D. Hamilton, Lopa Leach
    Methods in Molecular Biology

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Human/Primate VEGF165 Biotinylated Antibody
By Anonymous on 11/16/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Human

This antibody was used as the detection in an ELISA targeting VEGF in human serum samples.

ELISA Human/Primate VEGF<sub>165</sub> Biotinylated Antibody BAF293