Human PlGF Antibody

Catalog #: AF-264-PB
6 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF-264-PB
AF-264-SP

Detection of Human PlGF by Western Blot.

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Citations (6)

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Human PlGF Antibody Summary

Species Reactivity

Human

Specificity

Detects human PlGF (but not VEGF) in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human PlGF
Ala21-Arg149
Accession # CAA38698

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human PlGF by Western Blot. Western blot shows lysates of CHO Chinese hamster ovary cell line either mock transfected or transfected with full length human PlGF. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human PlGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-264-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PlGF at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PlGF

Placenta Growth Factor (PlGF) is a member of the Vascular Endothelial Growth Factor (VEGF) family of growth factors. PlGF and VEGF share primary structural as well as limited amino acid sequence homology with the A and B chains of PDGF. All eight cysteine residues involved in intra- and inter-chain disulfides are conserved among these growth factors. In their PDGF-like regions, VEGF and PlGF also share approximately 53% amino acid sequence similarity. The gene for PlGF has been mapped to chromosome 14. As a result of alternative splicing, at least two PlGF mRNAs encoding monomeric PlGF precursors containing 149 and 170 amino acid residues have been described. The expression of PlGF is not widespread, but has been detected in human umbilical vein endothelial cells, placenta, choriocarcinoma cell lines, and in renal cell carcinoma associated with angiogenesis. The PlGF proteins bind with high-affinity to Flt-1, but not to Flk-1/KDR.

Long Name

Placenta Growth Factor

Entrez Gene IDs

5228 (Human); 18654 (Mouse)

Alternate Names

D12S1900; PGF; PGFL; placenta growth factor; placental growth factor; placental growth factor, vascular endothelial growth factor-related protein; PlGF; PlGF-2; PLGFplacental growth factor-like; SHGC-10760

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human PlGF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Role of IL-36 Cytokines in the Regulation of Angiogenesis Potential of Trophoblast Cells
Authors: JM Murrieta-C, RN Gutiérrez-, HM El-Shorafa, T Groten, S Rodríguez-, ME Cancino-Di, JC Cancino-Di, RR Favaro, UR Markert, DM Morales-Pr
International Journal of Molecular Sciences, 2020-12-30;22(1):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Discovery of pan-VEGF inhibitory peptides directed to the extracellular ligand-binding domains of the VEGF receptors
Authors: Jussara S. Michaloski, Alexandre R. Redondo, Leila S. Magalhães, Caio C. Cambui, Ricardo J. Giordano
Science Advances
Placental growth factor neutralising antibodies give limited anti-angiogenic effects in an in vitro organotypic angiogenesis model.
Authors: Brave SR, Eberlein C, Shibuya M, Wedge SR, Barry ST
Angiogenesis, 2010-10-16;13(4):337-47.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
VEGF-A and VEGF-F evoke distinct changes in vascular ultrastructure.
Authors: Matsunaga Y, Yamazaki Y, Suzuki H, Morita T
Biochem. Biophys. Res. Commun., 2009-01-04;379(4):872-5.
Species: Guinea Pig
Sample Types: Whole Tissue
Applications: IHC
Malignant mesothelioma growth inhibition by agents that target the VEGF and VEGF-C autocrine loops.
Authors: Masood R, Kundra A, Zhu S, Xia G, Scalia P, Smith DL, Gill PS
Int. J. Cancer, 2003-05-01;104(5):603-10.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Vascular endothelial growth factor ligands and receptors that regulate human cytotrophoblast survival are dysregulated in severe preeclampsia and hemolysis, elevated liver enzymes, and low platelets syndrome.
Authors: Zhou Y, 2019, McMaster M, Woo K, Janatpour M, Perry J, Karpanen T, Alitalo K, Damsky C, Fisher SJ
2002-04-01;160(4):1405-23.
Species: Human
Sample Types: Whole Tissue
Applications: IHC