Human Plexin B1 APC-conjugated Antibody

Catalog #: FAB37491A Datasheet / COA / SDS

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FAB37491A

Detection of Plexin B1 in Jurkat Human Cell Line by Flow Cytometry.

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Human Plexin B1 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Plexin B1 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 559830

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human Plexin B1
Accession # O43157

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Plexin B1 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human Plexin B1 APC-conjugated Monoclonal Antibody (Catalog # FAB37491A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Plexin B1

Plexin B1 is a 300 kDa member of the B subfamily, Plexin family of Semaphorin receptors. Mature human Plexin B1 is a 2116 amino acid type I transmembrane (TM) glycoprotein that contains a 1471 aa extracellular domain (ECD) and a 612 aa cytoplasmic region. The ECD contains one Semaphorin domain, two PSI domains, and three IPT repeats. The ECD is cleaved into two subunits, a 200 kDa alpha -chain (aa 20-1305) and a 100 kDa TM beta -chain. The subunits are nondisulfide-linked and generate a high-affinity receptor. Plexin B1 is a receptor for Semaphorin 4D/CD100. It forms a receptor complex with neuropilins, MET, and EGF R2. Of three known splice variants, one shows a deletion of aa 689-871, a second shows a 53 aa substitution for aa 677‑2135, and a third shows a 53 aa substitution for the 53 aa between aa 677‑729. Over aa 35-150, human Plexin B1 shares 90% and 97% aa sequence identity with dog and mouse Plexin B1, respectively.

Entrez Gene IDs

5364 (Human); 235611 (Mouse); 316009 (Rat)

Alternate Names

Plexin B1; PLXN5; PLXNB1; SEP; Transmembrane Protein SEP