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Human Plexin B1 Antibody

Catalog #: MAB37491
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB37491
MAB37491-SP
Detection of Plexin B1 in Jurkat Human Cell Line by Flow Cytometry.
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Plexin B1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Plexin B1 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 559830
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human Plexin B1 Accession # O43157
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Plexin B1 antibody in Jurkat Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Plexin B1 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human Plexin B1 Monoclonal Antibody (Catalog # MAB37491, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Background: Plexin B1

Plexin B1 is a 300 kDa member of the B subfamily, Plexin family of Semaphorin receptors. Mature human Plexin B1 is a 2116 amino acid type I transmembrane (TM) glycoprotein that contains a 1471 aa extracellular domain (ECD) and a 612 aa cytoplasmic region. The ECD contains one Semaphorin domain, two PSI domains, and three IPT repeats. The ECD is cleaved into two subunits, a 200 kDa alpha -chain (aa 20-1305) and a 100 kDa TM beta -chain. The subunits are nondisulfide-linked and generate a high-affinity receptor. Plexin B1 is a receptor for Semaphorin 4D/CD100. It forms a receptor complex with neuropilins, MET, and EGF R2. Of three known splice variants, one shows a deletion of aa 689-871, a second shows a 53 aa substitution for aa 677-2135, and a third shows a 53 aa substitution for the 53 aa between aa 677-729. Over aa 35-150, human Plexin B1 shares 90% and 97% aa sequence identity with dog and mouse Plexin B1, respectively.

Entrez Gene IDs
5364 (Human); 235611 (Mouse); 316009 (Rat)
Alternate Names
Plexin B1; PLXN5; PLXNB1; SEP; Transmembrane Protein SEP

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Citation for Human Plexin B1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Semaphorin 4D is upregulated in neurons of diseased brains and triggers astrocyte reactivity
    Authors: EE Evans, V Mishra, C Mallow, EM Gersz, L Balch, A Howell, C Reilly, ES Smith, TL Fisher, M Zauderer
    Journal of Neuroinflammation, 2022-08-06;19(1):200.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay

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