Human Plexin B1 Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Plexin B1 in Human Brain. Plexin B1 was detected in immersion fixed paraffin-embedded sections of human brain using Mouse Anti-Human Plexin B1 Monoclonal Antibody (Catalog # MAB3749) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Background: Plexin B1
Plexin B1 is a 300 kDa member of the B subfamily, Plexin family of Semaphorin receptors. Mature human Plexin B1 is a 2116 amino acid type I transmembrane (TM) glycoprotein that contains a 1471 aa extracellular domain (ECD) and a 612 aa cytoplasmic region. The ECD contains one Semaphorin domain, two PSI domains, and three IPT repeats. The ECD is cleaved into two subunits, a 200 kDa alpha -chain (aa 20-1305) and a 100 kDa TM beta -chain. The subunits are nondisulfide-linked and generate a high-affinity receptor. Plexin B1 is a receptor for Semaphorin 4D/CD100. It forms a receptor complex with Neuropilins, MET, and EGF R2. Multiple splice variants are known. One shows a deletion of aa 689-871. A second shows a 53 aa substitution for aa 677-2135. And a third shows a 53 aa substitution for the 53 aa between aa 677-729. Over aa 35-150, human Plexin B1 is 90% and 97% aa identical to dog and mouse Plexin B1, respectively.
Product Datasheets
Citations for Human Plexin B1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Guidance landscapes unveiled by quantitative proteomics to control reinnervation in adult visual system
Authors: N Vilallongu, J Schaeffer, AM Hesse, C Delpech, B Blot, A Paccard, E Plissonnie, B Excoffier, Y Couté, S Belin, H Nawabi
Nature Communications, 2022-10-13;13(1):6040.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Grb2 mediates semaphorin-4D-dependent RhoA inactivation.
Authors: Sun, Tianlian, Krishnan, Rameshku, Swiercz, Jakub M
J Cell Sci, 2012-04-14;125(0):3557-67.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
ErbB-2 signals through Plexin-B1 to promote breast cancer metastasis.
Authors: Worzfeld T, Swiercz JM, Looso M
J. Clin. Invest., 2012-03-01;122(4):1296-305.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation
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