Human Plasminogen Antibody

Catalog #: MAB1939
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1939
MAB1939-SP

Neutralization of Plasminogen Activity by Human Plasminogen Antibody.

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All Plasminogen Antibodies

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Plasminogen Antibody Summary

Species Reactivity

Human

Specificity

Detects human PLG, human Plasmin, and the catalytic domain in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 270409

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human plasma-derived Plasminogen
Glu20-Asn810

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Human Plasminogen (Catalog # 1939-SE), see our available Western blot detection antibodies

Neutralization

Measured by its ability to neutralize Human Plasminogen (1 µg/mL, Catalog # 1939-SE) cleavage of the fluorogenic peptide substrate Suc-AFK-Amc (100 µM). The Neutralization Dose (ND₅₀) is typically 1 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Neutralization of Plasminogen Activity by Human Plasminogen Antibody. The cleavage of Suc-AFK-AMC (100 µM) by Human Plasminogen (1 µg/mL, Catalog # 1939-SE) is measured after preincubation with increasing concentrations of Mouse Anti-Human Plasminogen Monoclonal Antibody (Catalog # MAB1939). The ND₅₀ is typically 1 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Plasminogen

Plasminogen (PLG) is the precursor of plasmin, an active serine protease that dissolves the fibrin of blood clots and acts in many other processes such as embryonic development, tissue remodeling, inflammation, and tumor invasion (1, 2). Synthesized in the kidney, PLG is found in plasma and many extracellular fluids. Activated by u- or t-plasminogen activator, the single-chain PLG (amino acid residues 20-810) is converted to plasmin, which consists of disulfide bond-linked heavy chain A (residues 20-580) and light chain B (residues 581-810). Heavy chain A contains 5 kringle domains and light chain B corresponds to the serine protease domain. A fragment consisting of the first 4 kringle domains has been named as angiostatin, a novel angiogenesis inhibitor (3, 4).

References

Petersen, T.E. et al. (1990) J. Biol. Chem. 265:6104.
Forsgren, M. et al. (1987) FEBS Lett. 213:254.
O’Reilly, M.S. et al. (1994) Cell 79:315.
Sim, B.K. et al. (1997) Cancer Res. 57:1329.

Entrez Gene IDs

5340 (Human); 18815 (Mouse); 85253 (Rat)

Alternate Names

DKFZp779M0222; EC 3.4.21; EC 3.4.21.7; plasmin; Plasminogen; Plg

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Related Research Areas

Citations for Human Plasminogen Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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