Human Phospho-RelA/NFkB p65 (S536) Antibody Summary
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of Human Phospho-RelA/NF kappa B p65 (S536) by Western Blot. Western blot shows lysates of HT-29 human colon adenocarcinoma cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 100 nM Calyculin A (Catalog # 1336) and 20 ng/mL Recombinant Human TNF-a (Catalog # 210-TA) for 10 minutes. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Phospho-RelA/NF kappa B p65 (S536) Monoclonal Antibody (Catalog # MAB7226) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospho-RelA/ NF kappa B p65 (S536) at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Phospho-RelA/NFkB p65 (S536) in HT‑29 Human Cell Line. RelA/NF kappa B p65 phosphorylated at S536 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line untreated (lower panel) or treated with Calyculin A (Catalog # 1336) and Recombinant Human TNF-a (Catalog # 210-TA; upper panel) using Mouse Anti-Human Phospho-RelA/NF kappa B p65 (S536) Monoclonal Antibody (Catalog # MAB7226) at 2 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Preparation and Storage
Background: RelA/NFkB p65
RelA belongs to a family of transcription factors (NF kappa B (nuclear factor kappa from B cells) complex) that play a fundamental role in inflammatory and immune responses. The NF kappa B complex is composed of a heterodimer of a Rel family member (RelA, c-Rel, RelB) and either NF kappa B1 or NF kappa B2 subunits. RelA and NF kappa B1 are the most common heterodimeric pair. The NF kappa B complex is sequestered in the cytoplasm by inhibitory I kappa B proteins. Upon cellular activation, the ubiquitin-proteosome pathway degrades the I kappa B proteins allowing the NF kappa B complex to translocate to the nucleus and activate gene transcription.
Product Datasheets
Citations for Human Phospho-RelA/NFkB p65 (S536) Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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beta1-Integrin plays a major role in resveratrol-mediated anti-invasion effects in the CRC microenvironment
Authors: A Brockmuell, AL Mueller, P Shayan, M Shakibaei
Frontiers in Pharmacology, 2022-09-02;13(0):978625.
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Evidence That beta 1-Integrin Is Required for the Anti-Viability and Anti-Proliferative Effect of Resveratrol in CRC Cells
Authors: Aranka Brockmueller, Parviz Shayan, Mehdi Shakibaei
International Journal of Molecular Sciences
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Resveratrol Modulates Chemosensitisation to 5-FU via beta1-Integrin/HIF-1alpha Axis in CRC Tumor Microenvironment
Authors: A Brockmuell, S Girisa, AB Kunnumakka, M Shakibaei
International Journal of Molecular Sciences, 2023-03-05;24(5):.
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Calebin A targets the HIF-1?/NF-?B pathway to suppress colorectal cancer cell migration
Authors: Brockmueller, A;Girisa, S;Motallebi, M;Kunnumakkara, AB;Shakibaei, M;
Frontiers in pharmacology
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
Protective effect of KLF15 on vascular endothelial dysfunction induced by TNF??.
Authors: Bing Liu, Lili Xu, Xinming Yu, Wei Li, Xiaozhi Sun, Shun Xiao, Mingjin Guo, Haofu Wang
Molecular Medicine Reports, 2018-06-20;0(0):1791-3004.
Species: Human
Sample Types: Cell Lysate, Cell Lysates
Applications: Western Blot
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