Human PDK-1 Antibody

Catalog #: AF864 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF864
AF864-SP

Detection of Human PDK‑1 by Western Blot.

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Human PDK-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human PDK-1 in Western blots.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

KLH-coupled human PDK-1 synthetic peptide
CKIIKLEYDFPEKFFPKARD
Accession # AAD38505

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human PDK‑1 by Western Blot. Western blot shows lysates of WS-1 human fetal skin fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human PDK-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF864) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for PDK-1 at approximately 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDK-1

PDK-1 (Phosphoinositide-dependent Kinase-1), also called PKB kinase and PDPK1, is a member of the AGC group of serine/threonine kinases. Following insulin, growth factor, or pervanadate stimulation, PDK-1 phosphorylates and activates kinases from the Akt, p70 S6K, and RSK families, and is therefore involved in the regulation of cell proliferation and apoptosis.

Long Name

Phosphoinositide Dependent Kinase-1

Entrez Gene IDs

5170 (Human); 18607 (Mouse); 81745 (Rat)

Alternate Names

3-phosphoinositide-dependent protein kinase 1; 3-phosphoinositide-dependent protein kinase 2 pseudogene; PDK1; PDK-1; PDPK1; PDPK2; PDPK2P; PkB kinase like gene 1; PKB kinase