Human PDGF R alpha APC-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of PDGF R alpha in U‑118‑MG Human Cell Line by Flow Cytometry. U-118-MG human glioblastoma/astrocytoma cell line was stained with Mouse Anti-Human PDGF Ra APC-conjugated Monoclonal Antibody (Catalog # FAB1264A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: PDGF R alpha
PDGF is a major serum mitogen that can exist as a homo- or heterodimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA, PDGF‑BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Whereas PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF‑BB and AB, but not PDGF-AA. Both PDGF R alpha and PDGF R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and heterodimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Only PDGF R alpha is expressed in oligodendrocyte progenitor cells, mesothelial cell and liver endothelial cells. Soluble PDGF-R alpha has been detected in cell conditioned medium and human plasma. Recombinant soluble PDGF R alpha binds PDGF with high affinity and is a potent PDGF antagonist (1).
- Heldin, C.H. and L. Claesson-Welsh (1994) Guidebook to Cytokines and Their Receptors, Nicola, N.A. (ed) Oxford University Press, New York, NY p. 202.
Product Datasheets
Citations for Human PDGF R alpha APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
10
Citations: Showing 1 - 10
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Development of a scalable suspension culture for cardiac differentiation from human pluripotent stem cells
Authors: Vincent C. Chen, Jingjing Ye, Praveen Shukla, Giau Hua, Danlin Chen, Ziguang Lin et al.
Stem Cell Research
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Engineered 3D Immuno-Glial-Neurovascular Human Brain Model
Authors: Stanton, AE;Bubnys, A;Agbas, E;James, B;Park, DS;Jiang, A;Pinals, RL;Truong, N;Loon, A;Staab, C;Liu, L;Cerit, O;Wen, HL;Kellis, M;Blanchard, JW;Langer, RS;Tsai, LH;
bioRxiv : the preprint server for biology
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Gene editing to prevent ventricular arrhythmias associated with cardiomyocyte cell therapy
Authors: S Marchiano, K Nakamura, H Reinecke, L Neidig, M Lai, S Kadota, F Perbellini, X Yang, JM Klaiman, LP Blakely, E Karbassi, PA Fields, AM Fenix, KM Beussman, A Jayabalu, FA Kalucki, A Futakuchi-, GJ Weber, S Dupras, H Tsuchida, L Pabon, L Wang, BC Knollmann, S Kattman, RS Thies, N Sniadecki, WR MacLellan, A Bertero, CE Murry
Cell Stem Cell, 2023-04-06;30(4):396-414.e9.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Stage-specific regulation of DNA methylation by TET enzymes during human cardiac differentiation
Authors: Y Lan, KM Banks, H Pan, N Verma, GR Dixon, T Zhou, B Ding, O Elemento, S Chen, D Huangfu, T Evans
Cell Reports, 2021-12-07;37(10):110095.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Flow Cytometry, ICC, Western Blot -
Specific induction and long-term maintenance of high purity ventricular cardiomyocytes from human induced pluripotent stem cells
Authors: H Fukushima, M Yoshioka, M Kawatou, V López-Dávi, M Takeda, Y Kanda, Y Sekino, Y Yoshida, JK Yamashita
PLoS ONE, 2020-11-02;15(11):e0241287.
Species: Mouse
Sample Types: Whole Cells
Applications: MACS -
Physiologic expansion of human heart-derived cells enhances therapeutic repair of injured myocardium
Authors: S Mount, P Kanda, S Parent, S Khan, C Michie, L Davila, V Chan, RA Davies, H Haddad, D Courtman, DJ Stewart, DR Davis
Stem Cell Res Ther, 2019-11-04;10(1):316.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Chromatin and Transcriptional Analysis of Mesoderm Progenitor Cells Identifies HOPX as a Regulator of Primitive Hematopoiesis
Authors: NJ Palpant, Y Wang, B Hadland, RJ Zaunbreche, M Redd, D Jones, L Pabon, R Jain, J Epstein, WL Ruzzo, Y Zheng, I Bernstein, A Margolin, CE Murry
Cell Rep, 2017-08-15;20(7):1597-1608.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Cloche is a bHLH-PAS transcription factor that drives haemato-vascular specification
Nature, 2016-07-14;535(7611):294-8.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Generation of articular chondrocytes from human pluripotent stem cells.
Authors: Craft A, Rockel J, Nartiss Y, Kandel R, Alman B, Keller G
Nat Biotechnol, 2015-05-11;33(6):638-45.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Chromatin compartment dynamics in a haploinsufficient model of cardiac laminopathy.
Authors: Bertero A, Fields P A et al.
J Cell Biol
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